摘要
目的 了解T淋巴细胞亚群、B细胞及自然杀伤细胞 (NK细胞 )在活动期SLE患者中的改变 ,探讨这些免疫细胞在SLE发病机理中的作用。方法 采用微量全血直接双标记免疫荧光染色和流式细胞仪 ,对 3 8例活动期SLE患者及 2 0例正常人的外周血淋巴细胞表型进行检测。结果 3 8例活动期SLE患者CD+ 4细胞和CD+ 4/CD+ 8比值降低 (P <0 0 1) ,CD+ 3 细胞、CD+ 8细胞与正常对照组比较无统计学差异 (P >0 0 5 ) ,CD+ 19细胞 (B细胞 )升高 (P <0 0 0 1) ,而CD+ 16CD+ 56细胞 (NK细胞 )明显降低 (P <0 0 0 1)。结论 SLE患者存在免疫功能紊乱。T淋巴细胞亚群的改变和NK细胞活性降低 ,导致B细胞功能亢进 ,产生多种自身抗体 。
Objective To investigate the significance of changes in T lymphocyte subsets, B cells and natural killer cells (NK cells) of peripheral blood in patients with systemic lupus erythematosus (SLE). Methods Phenotypic analysis of peripherial blood lymphocytes was conducted in 38 cases of active SLE and 20 healthy controls by using doublestaining immunofluoresence and flow cytometry. Results Decrease in CD + 4 cells and CD + 4/CD + 8 was observed in SLE patients ( P<0.01 ). No statistically significant differences were shown between two groups in CD + 3 cells and CD + 8 cells ( P>0.05 ). CD + 16 CD + 56 cells (NK cells) significantly decreased and CD + 19 cells(B cells) significantly increased in SLE patients, as comparing with the control group ( P<0.001 ). Conclusion Immunologic disorder is present in the peripheral blood of SLE patients. The changes of peripheral T lymphocyte subsets and decrease of NK cells, which lead to proliferation of B cells, may play an important role in the development of SLE.
出处
《广东医学》
CAS
CSCD
2000年第5期383-384,共2页
Guangdong Medical Journal
关键词
系统性红斑狼疮
T细胞亚群
NK细胞
Lupus erythematosus, systemic\ T lymphocyte subsets\ NK cells\ Flow cytometry
