摘要
了解新疆石河子地区汉族结核分枝杆菌基因型特征、"北京家族"基因型的分布及其与耐药的相关性。应用多位点可变串联重复序列(VNTR)技术、RD105缺失检测法分析结核分枝杆菌的基因型,聚类分析采用Bionum-berics5.0软件分析。55株结核分枝杆菌临床分离株中,"北京家族"基因型47株;24位点VNTR检测结果显示明显的基因多态性,经基因聚类分析,可分为7个基因簇和46个独立的基因型,分为8个基因群(Ⅰ-Ⅷ群),其中46株属于Ⅵ群;耐药菌株为19株(34.5%)。新疆石河子地区结核分枝杆菌有明显的基因多态性;"北京家族"基因型为主要流行株,应引起重视;"北京家族"基因型与耐药无明显相关性。
To understand the genotyping characteristics of Mycobacterium tuberculosis （MTB） isolated from Han people in Shi- hei,Xinjiang,and identify the distribution of Beijing family genotype in this area and the correlation with drug-resitance. Variable numbers of tandem repeat （VNTR） and RD105 deletion test were used to identify MTB genotypes. The characteristics of polymorphism of DNA fingerprinting of MTB strains were analyzed with BioNumerics 5.0 software. 47 out of 55 MTB isolates were identified as Beijing family genotype. Twenty--four MLVA loci VNTR analysis revealed obvious polymorphisms in 55 MTB strains. Cluster analysis for gene expression showed that these strains could be categorized into eight gene groups（ Ⅰ -Ⅷ）. with 46 group Ⅵ isolates. Nineteen isolates （34.5 % ） were drug resistance. There was obvious gene polymorphism in MTB isolates in Shihezi;＂Beijing family＂ genotype was predominant in Han people in this area;and ＂Beijing family＂ genotype has no obvious correlation with drug--resistance.
出处
《石河子大学学报（自然科学版）》
CAS
2012年第2期220-224,共5页
Journal of Shihezi University(Natural Science)
基金
国家自然科学基金项目(30960356
81160368)
