摘要
目的：探讨诱导型一氧化氮合酶在血液动力学调控中的作用。方法：本实验所用动物为健康、雄性、Ｄａｈｌ盐敏感（ＤＳ）及 Ｄａｈｌ盐不敏感（ＤＲ）大鼠，体重（１９０±４）ｇ。通过慢性血液动力学实验观察静脉输入诱导型一氧化氮合酶（ｉＮＯＳ）抑制剂ＡＧ对大鼠平均动脉压的影响。此外，本实验还测定了一氧化氮（ＮＯ）终产物ＮＯ２－及ＮＯ３－含量以及钙依赖及钙不依赖的ＮＯＳ活性以了解ｉＮＯＳ的功能。结果：（１）ｉＮＯＳ特异抑制剂 ＡＧ对正常血压（包括高盐或低盐输入的Ｄａｈｌ盐抵抗大鼠及低盐输入的Ｄａｈｌ盐敏感大鼠）没有明显影响，但能明显放大高钠引起的ＤＳ大鼠的血压上升效应；（２）高盐在导致的ＤＳ大鼠血压升高的同时，能引起ｉＮＯＳ活性的明显升高。结论：ｉＮＯＳ是参与血液动力学调控的重要组成部分，尤其是在血压处于较高水平时，ｉＮＯＳ具有不容忽视的调节作用。
AIM and MEfTHODS: To clarify the role of inducible nitric oxide synthase (iNOS) in the regula- tion of blood pressure, in the present study, we examined the effect of aminoguanidine (AG), a selective inhibitor of iNOS on the hemodinamical response of Dahl salt - sensitive (DS) and Dahl salt - resistant (DR) rats to low (0. 3% ) or high (8%) sodium chloride (Nacl) infusion by chronical in vivo hemodynamic experiment, and the effect of NaCl or NaCl plus AG infusion on urinary nitrate (NO3)/nitrite (NO2), the end product of nitric oxide (NO),ex- cretion by Greiss Reaction. Furthermore, NOS activity assay was the dried out to probe the effect of NaCl and AG on calcium - dependent or independent NOS activity in renal tissue. RESULTS:1. High or low NaCl - infused DR rats and low NaCl - infused DS rats have no hemodinamical response to AG, however, the hpertensive effect of high NaCl (8% ) infusion on DS rats were gnatly amplified by co - infusion of AG. 2. Administration of high NaCl signif- icantly elevated the iNOS activity of renal tissue, and greatly increased urinary NO3/NO2 excretion. CONCLUSION: Ihducthle NOS is an important modulator of arterial pressure, especially in case of higher blood pressure.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2000年第5期397-400,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金(No.39870359)
教育部留学回国人员科研启动基金资助
