摘要
目的 克隆人肺腺癌细胞单羧酸转运泵基因编码序列并对此序列作同源性分析。方法 通过RT PCR方法克隆人肺腺癌A5 49细胞单羧酸转运泵基因cDNA编码序列 ,应用PCR、四色荧光、双脱氧终止法测序 ;通过Genebank数据库应用分析软件对克隆基因序列进行同源性分析。结果 应用RT PCR方法克隆出人肺癌细胞的单羧酸转运泵基因 ;肺癌细胞此基因编码序列与人心肌细胞单羧酸转运泵基因第一亚型的cDNA编码序列具有高度同源性。
Objective To clone the monocarboxylate transporter gene and analyze the homologous features of its sequence. Methods The code sequence of monocarboxylate transpoter cDNA of human adenocarcinoma A549 cells was cloned with RT PCR and sequenced with PCR, 4 fluorescent color test and Sanger′s method. The homologous features of the sequence was analyzed with the Genebank database. Results The monocarboxylate transporter gene of human pulmonary adenocarcinoma cells was cloned and the code sequence of the gene is highly homologous with the code region of human cardiac cells MCTI. Conclusion The monocarboxylate transporter gene might be a conservatioe sequence.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2000年第5期407-409,共3页
Journal of Third Military Medical University
基金
国家自然科学基金资助项目!(39900067)
关键词
单羧酸转运泵同源性
克隆
序列分析
肺腺癌
monocarboxylate transporter gene
RT PCR
DNA sequencing
adenocarcinoma
