摘要
目的 研究人大隐静脉器官培养过程中血管紧张素转换酶 (ACE)mRNA表达的变化。方法 用内参照逆转录 聚合酶链反应 (RT PCR)研究大隐静脉器官培养 1 4d后ACEmRNA的表达。结果 对照组静脉内膜厚度为 (3 .5± 2 .0 ) μm ,ACEmRNA水平为 0 .347± 0 .1 58;培养 1 4d后内膜厚度为 (47.0± 9.3) μm ,ACEmRNA水平 0 .777± 0 .1 4 3 ,两组差异有显著性。
Objective\ To investigate the changes in angiotensin converting enzyme (ACE) mRNA expression in organ cultured human saphenous veins. Methods\ Reverse transcription polymerase chain reaction (RT PCR) was used to detect the ACE mRNA expression in 30 pre culture veins and veins cultured for 14 days. Results\ Human saphenous vein intimal thickness was increased significantly after the veins cultured for 14 days. Expression of ACE mRNA in the experimental group was 0.777±0.143 (n=30), significantly higher than in the control group 0.347±0.158(n=30, P<0.001). Conclusion\ The expression of ACE mRNA was increased significantly in organ cultured human saphenous veins.\;
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2000年第3期203-204,共2页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目! ( 396 70 6 85)
关键词
人大隐静脉
器官培养
血管紧张素转换酶
MRNA
Human saphenous vein
\ Organ culture
\ Intimal proliferation
\ Angiotensin-converting enzyme
