摘要
目的 探讨磁场对肿瘤细胞凋亡及其相关基因 Bcl- 2及 Bax蛋白表达的影响 ,揭示磁场抗癌作用的分子机制。方法 460只 SD大鼠右下肢皮下移植 Walker- 2 56瘤株 ,常规饲养 2周后 ,随机选择 40 0只分为荷瘤对照组和荷瘤实验组。实验组每日接受不同强度不同时间的磁场处理 ,持续 2周 ,观察瘤鼠存活率 ,然后处死 ,测量瘤体体积及湿重 ,最后行 HE及免疫组化染色。结果 与对照组比较 ,实验组大鼠存活率 (84.2 8% )增高 ,肿瘤体积 (1 .78cm3± 0 .82 cm3)及肿瘤湿重 (2 .2 0 g± 1 .43g)明显减小 (P <0 .0 5)。病理组织学检查可见细胞凋亡的数目明显增多。Bcl- 2蛋白阳性表达率 (1 8% )低于对照组 ,Bax蛋白阳性表达率 (43% )高于对照组 (P <0 .0 5) ,Bcl- 2 /Bax的比值低于对照组。结论 恒定均匀磁场的作用结果使凋亡相关基因 Bcl- 2蛋白的表达下调 ,Bax蛋白的表达增高 ,Bcl- 2 /Bax的比值减小 。
Objective To study the expression of Bcl 2 and Bax proteins,to explore the molecular mechanism of antitumor effect and supply data and vidences in the treatment of tumors on Walker 256 implanted rats following HSMF. Methods Walker 256 carcinoma cells line was transplated into the right thighs of 460 SD rats,400 of 460 samples were divided into 2 qroups randomly:the tumor experiment group and the tumor control group on the basis of completely random principle after being fed with 2 weeks on routine.The experiment group were exposed to differential power and time magetic field every day, to continue 2 weeks and observe the survival rate of rats, then to measure volume and wet weighty of the body of sarcoma, in the last HE and ABC immunohistochemistry staining method were used.Results In the period of experiment, it was found that SD rats surrival rate(84.28%) was increased ,sarcoma volume and wet weighty were significantly decreased when compared with the tumor control group (P<0.05).The pathology examination showed that the number of apoptosis were significantly increased.The positive expression rate (18%) of Bcl 2 protein were decreased, the expression of Bax gene was contrary to Bcl 2 gene and the ratio of Bcl 2/Bax was significantly decreased.There was significant differenced in 2 groups (P<0.05).Conclusion HSM can decrease the expression of Bcl 2 gene, increase the expression of Bax and decrease the ratio of Bcl 2/Bax.It suggested that it achieved its antitumor effect by inducing apoptosis of SD rats transplation sarcoma cells .HSM showed a potentiation of the antitumor effect as a clinically new useful tool.
出处
《西安医科大学学报》
CSCD
2000年第2期100-103,共4页
Journal of Xi'an Medical University(Chinese)
