摘要
目的 研究非小细胞肺癌组织中端粒酶活性表达 ,探讨端粒酶活性表达与非小细胞肺癌发生发展的关系。方法 收集经手术及病理证实的非小细胞肺癌 48例标本 ,1 2例肺癌癌旁肺组织、7例非肿瘤病例所取正常肺组织作对照。用端粒酶检测试剂盒检测组织标本端粒酶活性。结果 75 0 0 % ( 36 / 48)非小细胞肺癌组织标本检出端粒酶活性 ,8.33% ( 1 / 1 2 )癌旁肺组织检出端粒酶活性 ,7例非肿瘤标本所取的正常肺组织均未检出端粒酶活性。肺癌组织与癌旁及正常肺组织端粒酶活性阳性率差异有显著性 (P <0 .0 1 )。不同组织学分型的非小细胞肺癌 ,其端粒酶活性阳性率差异均无显著性。不同组织学分级、不同TNM分期的非小细胞肺癌组织端粒酶活性阳性率差异有显著性 ,中低分化的非小细胞肺癌端粒酶活性阳性率高于高分化非小细胞肺癌 (P <0 .0 5 ) ,Ⅱ和Ⅲ期高于Ⅰ期 (P =0 .0 40 4)。结论 端粒酶活化在非小细胞肺癌的发生发展中起重要作用 ,端粒酶活性阳性率与非小细胞肺癌的组织学分级和TNM分期有关 ,随组织学分级和TNM分期的增高 ,端粒酶活性阳性率增高。端粒酶活性有望成为预测肿瘤发生、肿瘤诊断的良好指标。端粒。
Objective To investigate the activity of telomerase in human nonsmall cell lung cancer (NSCLC) and the correlation of telomerase activity with the cancer development. Methods Totally 48 cases of NSCLC tissues were studied accompanied with 7 cases of normal lung tissues and 12 samples of lung tissues adjacent to the tumor. The telomerase activity was detected using Telomerase PCR ELISA kit. Statistics study included χ2test and the exact probabilities. The significance level chosen was P value less than 0.05 (P<0.05), and all tests were twosided. Results 75% (36/48) of NSCLC tissues possessed the expression of telomerase activity and only 8.33% (1/12) of tissues adjacent to tumor and none of the normal lung tissue enabled to express telomerase activity. The statistical difference was significant (P<0.05). No significant difference was found between diffenernt histological types of NSCLC, however, the frequency of telomerase expression differed significantly between tumors of well/mordeately differentiated and poorly differentiated; stage Ⅰ and stage Ⅱ/Ⅲ. Conclusion Telomerase activity might be a good marker in estimating the occurrence, development and diagnosis of NSCLC.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
2000年第2期89-91,共3页
Chinese Journal of Pathology
基金
国家自然科学基金资助项目!(39770304)
