摘要
研究氧化苦参碱 (oxym atrine ,OM)对脂多糖 (LPS)加氨基半乳糖 (GalN)诱导的小鼠暴发型肝衰竭 (ful minanthepaticfailure ,FHF)的保护作用及其机理。实验分三组 :OM组 (10 0mgkg-1d-1× 3,ip) ,模型组及正常组。注射GaIN/LPS后 7.5小时 ,检测小鼠血清丙氨酸氨基转移酶 (ALT)和肿瘤坏死因子α(TNF -α)水平 ,同时取肝组织作HE染色和Fas及其配体 (FasL)的免疫组化检测。OM组小鼠ALT及TNF α水平明显低于模型组 (P <0 .0 1和 0 .0 5 )。OM组肝损害程度及Fas、FasL表达强度均较模型组减轻 (P <0 .0 1)。OM可能通过抑制TNF -α活性及Fas、FasL表达 。
The experiment aimed to study the protective effect and its mechanism of oxymatrine(OM)on the fulminant hepatic failure(FHF)induced by injecting lipopolysaccharide(LPS)in galactosamine(GalN)sensitized mice.Thirty mice were divided into three groups:OM group(100mgkg -1 d -1 ×3,ip),model group and normal group.The serum levels of alanine aminotransferase(ALT)and tumor necrosis factor alpha(TNF α)in all mice were determined at 7.5 hours after injecting GaIN/LPS.At the same time,the liver tissues were takan for HE staining and the immunohistochemical detection of Fas and its ligand(FasL).Results:The serum levels of ALT and TNF α in mice of OM group were decreased significantly than that of model group(P<0.05 and 0.01,respectively).Both the degrees of liver injury and expression of Fas and FasL in mice of OM group were reduced obviously than that of model group(P<0.01).Conclution:It is possible that OM blocks the hepatocyte apoptosis and necrosis induced by LPS through inhibiting the activity of TNF α and the expression of Fas and FasL in liver tissue.
出处
《临床肝胆病杂志》
CAS
北大核心
2000年第2期92-93,共2页
Journal of Clinical Hepatology
关键词
氧化苦参碱
暴发型肝功能衰竭
小鼠
Oxymatrine
Lipopolysaccharide
Fulminant hepatic failure
Fas antigen
Fas ligand
