摘要
以曙红Y-DNA为荧光探针,运用紫外可见光谱法、荧光光谱法和电泳法研究了纳米银与曙红Y-DNA之间的共振能量转移,并应用能量共振转移的原理解释了纳米银及曙红Y与DNA之间的相互作用。结果表明,曙红Y与DNA的作用方式为嵌插作用,纳米银与DNA的作用方式为静电作用,曙红Y和纳米银结合到DNA上以后,导致了DNA结构的改变。
The resonance energy transfer between nano-Ag and eosin Y （EY）-DNA was studied by UV-Vis spectrophotometry, fluorospectrophotometry and electrophoresis with EY-DNA as fluorescence probe. The phenomenon of interaction between nano-Ag and EY-DNA was explained by the theory of fluorescence resonance energy transfer （FRET）. It was found that the reaction of EY with DNA was due to inserting action, and the reaction between nano Ag and DNA was due to electrostatic action. The structure of DNA was changed after binding with EY and nano-Ag.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2012年第6期643-645,648,共4页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
山东省自然科学基金(ZR2010BL026和ZR2011BL026)
山东省高校科技计划(J09LC66)共同资助项目
