摘要
以大豆品种绥农14为材料,采用盆栽试验研究了大豆花期干旱胁迫条件下喷施不同浓度外源脱落酸(ABA)对大豆生理特性的影响,并对不同浓度脱落酸的作用效果进行比较。结果表明:与正常供水相比,干旱条件下的大豆叶片过氧化物酶活性、丙二醛含量、可溶性糖含量、游离脯氨酸含量均增加,超氧化物歧化酶活性和叶绿素含量降低,喷施一定浓度外源ABA明显缓解了干旱胁迫下大豆各项生理指标的变化幅度。喷施ABA后1～13 d,4.0 mg.L-1的ABA明显提高了过氧化物酶的活性、1.0 mg.L-1ABA能明显促进超氧化物歧化酶活性升高和可溶性糖含量增加,并能缓解叶绿素含量的降低;3.0 mg.L-1的ABA明显使脯氨酸含量增加;2.0 mg.L-1的ABA对缓解丙二醛积累作用明显。综合分析表明,干旱胁迫下,叶面喷施一定浓度的脱落酸维持了大豆花期叶片的正常生理代谢功能,有效的提高了叶片抗氧化能力和控制了叶片的衰老进程。
Soybean cv.Suinong 14 were pot planted in greenhouse,and different drought stress were set at flowering,then 1.0-8.0 mg·L-1 Abscisic acid（ABA）were leaf sprayed and content of superoxide dismutase（SOD）,peroxidase（POD）,proline,malondialdehyde（MDA）,soluble sugar and chlorophyll in soybean blades under drought stress were determined 1-13 days later.The results showed that compared with normal water irrigation,POD activity,content of soluble sugar and free proline in leaves under drought conditions increased.SOD activity and chlorophyll content decreased.Exogenous ABA relieved the change of these physiological indexes under drought stress.In the test,4.0 and 3.0 mg·L-1 ABA obviously improved POD activity and proline content,respectively;1.0 mg·L-1 ABA increased the soluble sugar content and SOD activity and alleviated the decrease of leaf chlorophyll content,2.0 mg·L-1 ABA alleviated the accumulation of MDA apparently.Above results indicated that a certain concentration of ABA could effectively enhance the antioxidant capacity and slow down the senescence of soybean leaves.
出处
《大豆科学》
CAS
CSCD
北大核心
2012年第3期385-388,394,共5页
Soybean Science
基金
黑龙江省科技攻关项目(GA09B103
GA09B104)
东北农业大学科技人才启动基金(2009RC57)
关键词
干旱胁迫
大豆
脱落酸
花期
生理特性
Drought stress
Soybean
Abscisic acid（ABA）
Flowering
Physiological characteristics
