摘要
目的:探讨人参皂苷Rg3对人宫颈癌Hela细胞的诱导凋亡作用。方法:培养人宫颈癌Hela细胞,不同浓度Rg3处理,HE染色观察细胞形态学改变,CCK-8、流式细胞仪检测细胞的凋亡。结果:Rg3作用后Hela细胞出现了典型的凋亡形态学变化,包括细胞皱缩、细胞核内染色体浓缩。10、20、40、80μg/ml人参皂苷Rg3处理48小时后Hela细胞的平均生存率为76.3%、57.1%、50.0%、29.8%;流式细胞术(FCM)显示肿瘤细胞凋亡率为16.4%、37.0%、50.0%、69.8%,表明肿瘤细胞在人参皂苷Rg3诱导下发生凋亡。结论:Rg3能抑制Hela细胞增殖,诱导其凋亡,其诱导凋亡作用呈剂量依赖性。
Objective： To investigate the apoptosis effect of ginsenoside Rg3 on cervical cancer Hela cells. Meth- ods： Hela cancer cells were treated with ascending concentration of ginsenoside Rg3. Morphologie changes of apop- totie cells were observed by optical microscope after HE staining. Cell survival rates and apoptosis were detected by cell counting kit -8 （CCK -8 ） and cell flow eytometry （FCM）. Results： Morphology changes of apoptosis and ap- optotic body were found by optical microscope, including cellular shrinkage and nucleus chromosome condensation. After treatment by ginsenoside Rg3 at dosage of 10,20,40,80μg/ml for 48 h, Hela cells were found to have a mean survival rate of 76.3% , 57. 1% , 50.0 % , 29.8 % , FCM showed a serious ascending apoptosis rates of 16.4%, 37.0% ;50.0% ,69.8%,demonstrating that Rg3 induced apoptosis in Hela cells. Conclusion： Rg3 can induce the apoptosis of Hela cells with a concentration dependent manner.
出处
《现代肿瘤医学》
CAS
2012年第7期1345-1347,共3页
Journal of Modern Oncology
基金
解放军总医院第一附属医院院管课题(编号:Y200927)
