摘要
目的 :探讨乙肝病毒免疫标志物常见模式与HBV -DNA量的关系。方法 :采用荧光定量PCR法对 15 0份测定的血清标本进行乙肝病毒标志物与HBV -DNA定量测定。结果 :2 1份HBsAg、HBeAg、HBcAb阳性标本 ,HBV -DNA全部阳性 ,平均拷贝数为 3.5× 10 8;6份HBsAg、HBeAg阳性的标本也均为阳性 ,平均拷贝数为 1.2× 10 9;2 7例HBsAg、HBeAb、HB cAb阳性的标本中HBV -DNA阳性 12例 ,平均拷贝数 7.5× 10 6;2 4例HBsAg、HBcAb阳性的模式中阳性 8例 ,平均拷贝数 8.5× 10 5;其它HBsAb阳性和全阴性血清HBV -DNA都呈阴性。结论 :提示FQ -PCR可以检测HBV的真实感染和复制状态 ,对于乙型肝炎的临床诊断 。
Objective:To study the relationship between the common mode of HBV immunological marker in serum and the quantity of HBV-DNA. Methods:150 samples were detected by fluorescence quantitative PCR (FQ-PCR) and ELISA kit respectively. Results:In 21HBsAg+/HBeAg+/HbcAb+samples, the FQ-PCR were all positive with 3.5×10 8copies on average,in 6 HBsAg+/HBeAg+samples,the HBV-DNA were all positive and the mean was 1.2×10 9copies. in 27 HBsAg+/HBeAb+/HBcAb+samples 12 samples were positive tested FQ-PCRwith 7.5×10 6 copies,on average; In 24 HBsAg+/HBcAb+samples , 8 samples were positive and the amount was 8.5×10 5, the residual HBsAb+and HBsAg -/HBsAb-/HBeAg-/HBeAb-/HBcAb- samples were negative by FQ-PCR. Conclusion:This indicates the FQ-PCR can be used to detect the true state of HBV infection and its replication. The FQ-PCR is helpful to diagnose the hepatitis B, and to select the therapeutic project and to monitor the medication.
出处
《重庆医科大学学报》
CAS
CSCD
2000年第2期169-170,173,共3页
Journal of Chongqing Medical University
