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HPV核酸探针斑点杂交技术在诊断尖锐湿疣中的应用 被引量：1

Dot hybridization technique in diagnosis of condyloma acuminata
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摘要 目的 :评估人类乳头状瘤病毒 (HPV)核酸斑点杂交技术在诊断尖锐湿疣中的价值。方法 :采用地高辛标记的HPV分型核酸探针斑点杂交技术 ,对 5 0例外阴、阴道尖锐湿疣和 2 6例保存的尖锐湿疣病理石蜡标本进行HPVDNA检测。结果 :5 0例临床标本阳性率为 84.0 % ,2 6例病理标本阳性率为 84.6 % ,差异无显著性 (P >0 .0 5 )。结论 :斑点杂交技术结合组织病理学检查对尖锐湿疣有诊断价值。 ?Objective:To evaluate DNA hybridization of HPV for detection of the condyloma acuminata.Methods:Fifty clinical external genital and vaginal specimens and 26 pathological sections from patients suspecting of condyloma acuminata were examined with dot hybridization technique by using Dig-HPV 6 and 11 gene probes.Results:The positive rate was 84.0% in clinical specimens and 84.6% in histological sections.Conclusions:The results in this study indicate that DNA hybridization together with histological examination are important for the diagnosis of condyloma acuminata. 〔
作者 夏佩莹 李凤云 汪万英 赵发英
机构地区 蚌埠医学院微生物学教研室 蚌埠医学院附属医院
出处 《蚌埠医学院学报》 CAS 2000年第3期163-164,共2页 Journal of Bengbu Medical College
关键词 尖锐湿疣 乳头状瘤病毒 核酸杂交 condyloma acuminata papillomaviruses nucleic acid hybridization
分类号 R752.530.4 [医药卫生—皮肤病学与性病学]
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蚌埠医学院学报
2000年 第3期

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