摘要
目的：分析中国人血清抗幽门螺杆菌（ＨＰ）抗体类型，探讨其与疾病的关系。方法：收集１４３例血清，应用蛋白质印迹方法检测抗体类型。结果：患者血清中主要存在１２．８万，１１．６万，９．１万，８． ７万，６．８万，６．６万，６．２万和３．５万等多条抗体带，其中以Ｈｐ ６．６万最多见，高达９１％。良性胃病与胃癌血清中前４条抗体带存在状态差别；但胃炎和消化性溃疡的血清类型无明显差别。结论：蛋白质印迹方法检测抗Ｈｐ抗体类型有助于良性胃病与胃癌的鉴别诊断。
Objective: To analyse the profile of serum antibodies against helicobacter pylori (HP) antigen and discover its correlation with Hp-associated diseases. Methods: Sera were collected from 143 patients with Helicobacter pylori infection. Antibody patterns were defined using immunoblot assay. Results: Immunoreactive bands at 128 000, 116 000, 91 000, 87 000, 68 000,66 000 62 000and 35 000 were presented in 143 human sera. The frequence of in sera was as high as 91 %. The frequencies of 128 000, 116 000, 91 000,and 87 000 were significantly different in patients with benign gastric diseases and gastric cancer, and not significantly different with gastritis and ulcer. Conclusion: Differential diagnosis of benign gastric diseases from gastric cancer can be made using immunoblot assay.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2000年第4期343-345,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金资助项目!(39670648)
关键词
幽门螺杆菌
抗体
蛋白质印迹法
胃疾病
helicobacter pylori
antibody
blotting westem
gastric disease
