摘要
目的：阐明慢性乙型肝炎中是否也存在肝特异性自身抗体及其在病情转归中的作用。方法：以猴肝组织切片和可溶性肝抗原为抗原基质，用间接免疫荧光和蛋白质印迹法检测３３７例慢性乙型肝炎和肝硬化患者血清中的自身抗体。结果：免疫荧光法显示乙型肝炎后肝硬化自身抗体总的阳性率（２４．０７％）高于慢性乙型肝炎组（１４．２９％），且两组均显著高于正常对照组。蛋白质印迹的阳性率各为４３．４２％和４２．５９％，两者无显著差异。两组病例靶抗原相对分子质量多集中在３．０万～５．５万之间，但肝硬化患者在相对分子质量２．５万和９．０万左右处的阳性条带均多于慢性肝炎患者。结论：慢性肝炎、肝硬化患者血清中存在抗肝组织自身抗体，其相对应的靶抗原有多种。进一步分离、鉴定有意义的靶抗原，并对其进行抗原表位、细胞表面表达等分析，将有助于探讨自身免疫在慢性肝炎发病及转归中的作用。
Objective: To investigate the effect of anti-liver tissueautoantibodies on development and worsening of chronic liver diseases. Methods: Autoantibodies were detected with indirect immunofluoresence and Western blot on monkey liver substrates and solubility liver-antigen. Results: The frequency of autoantibodies was significantly higher in 162 cases cirrhosis (24. 07 % ) than in 175 cases chronic hepatitis B(14. 29% ). The result of Western blot showed that the frequency of autoantibodies was similar in 2 groups, the molecular mass of liver-antigen was between 30 000 and 55 000, and there were more positive bands of about 25 000 and 90 000 in cirrhosis than in chronic hepatitis. Conclusion:There are autoantibodies in chronic hepatitis and cirrhosis, and some liver-antigens of 2 groups patients are different. It may play a role in investigating the effect of autoimmune on development and worsening of chronic hepatitis.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2000年第4期309-311,共3页
Academic Journal of Second Military Medical University
基金
卫生系统百名跨世纪优秀学科带头人培养计划资助基金![沪卫科(1999)21号]
