摘要
目的探讨在抗ＴＯＲＣＨ－ＩｇＭ型抗体检测中排除ＲＦ和特异ＩｇＧ产生的假阳性和假阴性结果的方法。方法在抗ＴＯＲＨ－ＩｇＭ型ＥＬＩＳＡ试剂的研究过程中．用羊抗人ＩｇＧ（γ特异）抗血清消除ＲＦ干扰。结果弱阳性ＯＤ_(４５０)下降不超过５０％而非特异性下降约８０％左右的情况下有效地消除了ＲＦ和特异ＩｇＧ的干扰．结论在调整好特异性和敏感性关系的前提下，用羊抗人ＩｇＧ（γ特异）抗血清，能有效地消除ＲＦ和特异ＩｇＧ的干扰，使弱阳性不漏检而假阳性被剔除。
Objective To prevent false negative and false positive IgM test results caused by the presence of rheumatoid factor(RF) and specific IgG in some specimens. Methods Sheep-anti-Human IgG (γ chain-specific) antibody was used to resolve interference by RF and specific IgG. Results and Conclusion The method can efficiently eliminate RF and specific IgG interference, decreasing OD_(450)of low positivity by 41.9% and non-specificity by 78.8%. False positive result was ruled out and low level of IgM can be tested.
出处
《第一军医大学学报》
CSCD
2000年第2期185-186,共2页
Journal of First Military Medical University
