摘要
目的:鉴别归芍胶囊中的白芍和测定归芍胶囊中芍药苷的含量。方法:采用薄层色谱法鉴别白芍,高效液相色谱法测定芍药苷的含量,色谱柱为Shimadzu VP-ODS(4.6 mm×150 mm,5μm),以乙腈-0.1%磷酸溶液(14∶86)为流动相、检测波长230 nm、柱温35℃。结果:归芍胶囊中白芍的鉴别方法和芍药苷的含量测定方法专属性强,在40～320 mg/L内线性关系良好,平均回收率为99.94%(RSD=1.15%)。结论:薄层色谱法鉴别归芍胶囊中白芍,HPLC法测定芍药苷含量的方法简便、灵敏、准确。
Objective： To identify Radix paeoniae alba and to determin paeoniflorin content in Guishao capsules. Methods： Radix paeoniae alba was identified with thin layer chromatography （TLC）. Paeoniflorin content was determined with high performance liquid chromatography （HPLC）. The HPLC method was carried out on Shimadzu VP-ODS （4.6 mm × 150 mm,5μm）column using acetonitrile -0.1% phosphoric acid（ 14： 86） as mobile phase Detection wavelength was 23Onto, and temperature of column was 35℃. Results： The identification method and the content determination method showed strong specificity. The calibration curve for paeoniflorin was linear in the range of 40 - 320 μg/ml. The average recovery was 99.94%, and RSI） was 1.15%. Conclusions： The methods are simple, sensitive and accurate.
出处
《贵阳医学院学报》
CAS
2012年第3期259-262,共4页
Journal of Guiyang Medical College
基金
贵州省中药现代化科技产业研究开发项目[黔科合中药专字(2003)74]
关键词
色谱法
薄层
色谱法
高压液相
白芍
芍药
chromatography, thin layer
chromatography, high pressure liquid
radix paeonta veichii
