摘要
目的探讨碱性磷酸酶组化染色在炎症性肠病鉴别诊断中的价值。方法应用BCIP/NBT组化染色检测34例克罗恩病(CD组)、36例溃疡性结肠炎(UC组)及15例非炎症性肠病(NIBD组,包括12例肠结核、2例细菌性肠道感染和1例缺血性结肠炎)肠黏膜中肠碱性磷酸酶表达水平。结果 CD组碱性磷酸酶组化染色阳性率显著高于UC组和NIBD组(85.29%vs.5.56%和0)(P<0.05)。碱性磷酸酶组化染色诊断CD的敏感性为93.55%,特异性为90.74%,阳性预测率为85.29%,阴性预测率为96.08%;其与CD病变部位、是否伴有肛周病变、活动度及病变类型无明显相关性(P>0.05)。结论碱性磷酸酶BCIP/NBT组化染色可以作为CD诊断的一个辅助指标。
Objective To evaluate the value of alkaline phosphatase histochemical staining in the differential diagnosis of inflammatory bowel disease. Methods The expression of alkaline phosphatase BCIP/NBT in intestinal mucosa was detected by alkaline phosphatase BCIP/NBT histochemical staining in 34 Crohn＇s disease（group CD）,36 ulcerative colitis（group UC） and 15 non- inflammatory bowel disease （group NIBD, including 12 intestinal tuberculosis, 2 bacterial intestinal infection and i ischemic colitis）. Results The BCIP/NBT alkaline phosphatase positive staining [BCIP/NBT（＋）] rate was higher in group CD than that in groups of UC and NIBD（85.29.0/o vs. 5.56%和10）（P〈0. 05）. The sensitivity, specificity, positive prediction rate and negative prediction rate of BCIP/NBT histochemical staining were 93.55 %, 90. 74%, 85.29% and 96.08%, respectively. There was no any significant correlation between BCIP/NBT（＋） and the location, perianal lesions, activity level and pathological category of CD（P〉0. 05）. Conclusion BCIP/NBT alkaline phosphatase histochemical staining can be taken as an useful assistant index in the diagnosis and differential diagnosis of CD.
出处
《江苏医药》
CAS
CSCD
北大核心
2012年第13期1543-1545,共3页
Jiangsu Medical Journal
基金
江苏省国家中医临床研究基地(脾胃病)开放课题(JD11013)
