摘要
目的 探讨血小板衍生生长因子 (PlateletderivedgrowthfactorPDGF)在瘢痕疙瘩形成中的作用。方法 采用免疫组织化学方法检测了 8例瘢痕疙瘩和 9例正常人皮肤对照中PDGF受体α和 β亚单位的表达。结果 9例正常人皮肤对照中仅 2例有α亚单位的微弱表达 ;3例有 β亚单位的微弱表达。 8例瘢痕疙瘩皮损的PDGF受体α和 β亚单位全部染色阳性 ,并且染色强度和阳性染色百分数都明显高于正常对照。统计学检验分析显示两组间有显著差异 (P〈0 .0 1)。结论 PDGF受体α和 β亚单位在瘢痕疙瘩皮损表达增强提示PDGF可能参与了瘢痕疙瘩的形成。
Objective To investigate the role platelet-derived growth factor(PDGF) in the development of keloid. Methods The expression of PDGF receptor α and β subunits were detected in 9 healthy subjects skin and 8 keloid patients lesions through immunohistochemistry staining.Results Among 9 healthy controls skin,2 specimens showed weak expression of PDGF receptor α subunit and 3 showed faint expression of PDGF receptor β subunit.Only a few fibroblasts and endotheliocyte were positively stained.Most of controls specimens were negative. All of 8 keloid specimens showed increased expressions of PDGF receptor α and β subunits.A great number of fibroblasts were positively stained.The staining density was more intensive and percentage of positive staining cell in keloid lesions were greater than these of in healthy control skins.The difference between these two groups were of statistical significance (P <0.01).Conclusion The findings of increased PDGF receptor α and β subunits indicated that PDGF may participate in the formation of keloid.
