摘要
【目的】探讨窖蛋白-1(caveolin-1)在新诊断2型糖尿病患者外周血单个核细胞(PBMC)中的表达水平,并分析其与胰岛素抵抗的关系。【方法】检测47例新诊断2型糖尿病患者和41例正常血糖对照者PBMC中caveolin-1 mRNA和蛋白的表达,分析caveolin-1 mRNA表达与体质指数(BMI)、腰围、臀围、腰臀比、空腹血糖(FPG)、糖化血红蛋白(HbA1C)、空腹胰岛素(FINS)、稳态模型的胰岛素抵抗指数(HOMA-IR)和血脂的相关性。【结果】新诊断2型糖尿病患者PBMC中caveolin-1 mRNA和蛋白水平均明显低于对照组(P<0.05),且其caveolin-1 mRNA水平与FPG、HbA1C、HOMA-IR及甘油三酯(TG)呈负相关(r分别为-0.376、-0.294、-0.309、-0.397,P均<0.05)。以caveolin-1 mRNA为因变量,年龄、性别、BMI、臀围、FPG、logFINS、logHOMA-IR、HbA1C、TG为自变量,进行多重线性逐步回归分析,结果显示HbA1C是caveolin-1 mRNA的独立影响因素。【结论】Caveolin-1在新诊断2型糖尿病患者PBMC中表达较正常血糖人群下降,其低表达与新诊断2型糖尿病高血糖及胰岛素抵抗相关。
[Objective] To investigate the caveolin-1 expression of peripheral blood mononuclear cells （PBMC） in newly diagnosed type 2 diabetic patients and its relationship with insulin resistance. [ Methods ] Caveolin-1 mRNA and protein expression of PBMC from 47 newly diagnosed type 2 diabetic patients and 41 euglycemic control subjects were compared, and the correlation between caveolin-1 mRNA expression with body mass index （BMI）, waist circumference （WC）, hip circumference （HC）, waist-to- hip ratio （WHR）, fasting plasma glucose （FPG）, glycated hemoglobin AIC （HbA1C）, fasting insulin （FINS）, homeostatic model assessment of insulin resistance （HOMA-IR） and lipid profiles were analyzed. [ Results ] Caveolin-1 mRNA and protein expression of PBMC were lower in TzDM group than in controls （P 〈 0.05）, furthermore, caveolin-1 mRNA expression in T2DM group was inversely correlated with FPG （r = -0.376, P 〈 0.01）, HbA1C（r = -0.294,P 〈 0.01）, HOMA-IR （r = -0.309, P 〈 0.01） and triglycerides （r = -0.397,P 〈 0.01）. Multiple linear stepwise regression analysis showed that HbA1C was an independently influencing factor of caveolin-1 mRNA expression. [Conclusion] Caveolin-1 mRNA and protein expression of PBMC is decreased in newly diagnosed type 2 diabetic patients which may contribute to higher glycemic levels and insulin resistance.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2012年第3期378-382,共5页
Journal of Sun Yat-Sen University:Medical Sciences
基金
国家自然科学基金(81070661/H0713)
