摘要
目的:对中国恒河猴感染猴免疫缺陷病毒(SIV)后CD4T初始、记忆亚群的细胞进行分析,了解各亚群的变化规律以及在AIDS发病过程中的意义。方法:使用SIVmac239毒株感染20只中国恒河猴,观察18个月;在感染前、后各时间点,使用实时荧光定量PCR(TaqMan探针法)进行病毒载量的检测;使用CD3/CD4/CD28/CD95表面标记的组合,进行CD4及其初始/记忆亚群比例的流式分析,并结合血常规检测的结果计算CD4及其各亚群的绝对数。结果:得到了CD4各个亚群在SIV感染后的变化曲线;并发现SIV感染后CD4减少的主体是中央型记忆(CM)CD4+T细胞;而初始型CD4+T在半数动物逐渐缓慢减少,但个体间差异较大。此外,还发现快速进展型RM449猴在死亡前其初始型及CM CD4仍较高,但其初始型CD4细胞在流式图上存在着类似"转化阻滞"的现象。结论:本研究展示了CD4细胞的初始、记忆亚群在感染SIV后的变化规律;对其进行细分研究,有助于更准确地了解体内免疫状态的变化,为临床疗效观察、免疫状态评估等提供参考。
Objective： To explore the dynamics of T-cell nave/memory subsets in SIV infected Chinese rhesus monkeys. Methods：20 Chinese rhesus monkeys were infected by SIVmac239,and their plasma viral load were determined by quantitative Taqman real-time RT-PCR analysis within a follow-up period of 18 months.Moreover,proportions of their CD4＋ T cells and its nave/memory subsets were analyzed by flow cytometry using a combination of surface markers CD3/CD4/CD28/CD95,by which the absolute cell counts of CD4 and its subsets were also computed based on the routine blood tests. Results： Dynamics of the T-cell nave/memory subsets including their percentages and absolute counts were illustrated.Moreover,central memory CD4 showed to be the majority of cell-loss after SIV infection,which decreased more significantly and accordantly than total CD4,and should be further evaluated as a better indicator for clinical surveillance and drugs judgement.Nave CD4 T cell also decreased gradually with disease progression in half of these animals with more variation among individuals.But impressively,1 monkey（RM449） with rapidly disease progression died with relatively higher nave and central memory CD4＋ T cells,which may be partially explained by the blockage of nave CD4 differentiation into effector memory CD4,as revealed by the flow cytometry assay. Conclusion： The dynamics of different subsets of CD4 T cells in Chinese rhesus monkeys infected with SIVmac239 were surveyed.These data are necessary for the further research of cellular anti-SIV immunity,and may be useful for the clinical judgement of immune reconstitution and vaccine evaluation in HIV/AIDS research.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2012年第6期483-488,共6页
Chinese Journal of Immunology
基金
国家科技重大专项(No.2008ZX10005-004)项目资助
关键词
猴免疫缺陷病毒
中国恒河猴
T细胞
记忆细胞亚群
Simian immunodeficiency virus （SIV）
Chinese rhesus monkey
T-cell
Memory subtypes
