摘要
目的建立谷芽α-淀粉酶激活剂(硝酸根离子和氯离子)的含量测定方法,并比较其不同炮制品中的含量。方法采用紫外分光光度法测定硝酸根,硝酸根在紫外光谱区有强烈的吸收,其吸光度与硝酸根的浓度成正比,可在210 nm波长处测定其吸光度。采用滴定法,用硝酸银标准溶液滴定氯离子,测定氯离子浓度。结果硝酸根质量浓度在0.1～5.0μg/mL范围内与吸光度线性关系良好(r=0.999 1),生谷芽、炒谷芽、焦谷芽中硝酸根离子含量分别为0.32%,0.15%,0.25%,氯离子的含量分别为0.016 9%,0.013 5%,0.008 0%。结论谷芽经过加热炒制后,其α-淀粉酶激活剂含量均有所下降。
Objective To establish two methods for the determination of alpha amylase activator（nitrate ions and chloride ions）in oryzae fructus germinatus（OFG）,and to compare it’s content in different processed products of OFG.Methods Using ultraviolet spectrophotometry for the determination of nitrate,the absorbance of nitrate was measured at 210 nm.Adopt titration,use nitrate standard solution titration chloride ions,determination of chloride ion concentration.Results A linear relationship was demonstrated over the range 0.1-5.0 μg/mL（r=0.999 1）.The content of the nitrate ions,which extracted from the crude product,the stir-fried to yellow product and the stir-fried to brown product of the OFG was 0.32%,0.15%and 0.25%respectively.The content of the chloride ions was 0.016 9%,0.013 5%and 0.008 0%.Conclusion The contents of the Alpha Amylase Activator in OFG fell after processing by frying.
出处
《中国药业》
CAS
2012年第12期18-19,共2页
China Pharmaceuticals
基金
2010年湖南省大学生研究性学习和创新性实验计划项目
项目编号:2010169
湖南省"十二五"重点学科中药学项目
项目编号:1008
关键词
谷芽
炮制品
α-淀粉酶激活剂
紫外分光光度法
含量
oryzae fructus germinatus
processed products
alpha amylase activator
UV spectrophotometry
content
