摘要
为了分析不同营养成分及培养条件对金针菇菌丝体生物量的影响及为金针菇液体发酵工业化生产提供参考依据,采用单因子及正交试验,分别测定不同培养基组合及培养条件下的金针菇菌丝体生物量.研究结果表明,金针菇液体发酵培养基的最优组合为:40 g/L黄豆粉、20 g/L可溶性淀粉、2 g/L KH2PO4和1 g/L MgSO4.7 H2O.最优培养条件是:起始pH值6.0、接种量200 mL/L、装液量400 mL/L、摇床转速160 r/min、培养温度25℃和恒温培养8 d.
The liquid fermentation for mycelium production by Flammulina velutipes is studied in this paper. The orthogonal and single-factor experiments are adopted to explore the influence of ingredients of medium and cultural conditions on the yielding biomasses of mycelia from Flammulina velutipes. The experimental results show that the compositions of optimal medium are as fellows： 40 g/L soybean flour, 20 g/L soluble starch,2 g/L KH2PO4 and 1 g/L MgSO4·7 H2O. The dry biomasses can reach maximum production with inoculated volumes 200 mL/L,culture volumes 400 mL/L,pH6.0,160 r/min,temperature 25 ℃,and cultivation eight days. The effective culture process for the high production of Flammulina velutipes in liquid fermentation is obtained with optimal medium and culture conditions.
出处
《文山学院学报》
2012年第3期12-15,共4页
Journal of Wenshan University
基金
滁州学院生物工程科技创新团队资助项目"真菌资源的开发利用研究"(CXTD201104)
滁州学院优秀中青年骨干教师研究经费资助
安徽省教育厅科研基金项目"大型真菌筛选及发酵生产工艺研究"(KJ2012Z287)
关键词
金针菇
液体发酵
菌丝体生物量
培养工艺
Flammulina velutipes
liquid fermentation
biomasses ofmycelia
culture process
