摘要
【目的】了解黑龙江省大豆田大豆胞囊线虫胞囊可培养细菌的多样性。【方法】运用稀释平板法和16SrDNA基因序列的系统发育分析对胞囊可培养细菌多样性进行研究。【结果】用NA培养基从胞囊上分离90株具有不同菌落形态的细菌。16S rDNA序列分析结果表明:90株菌株分属于7个属22个种。46株属于变形菌门γ亚群(Gammaproteobacteria),32株属于厚壁菌门(Firmicutes),10株属于变形菌门β亚群(Betaproteobacteria),2株属于变形菌门ɑ亚群(Alphaproteobacteria)。假单胞菌属(Pseudomonas)和芽孢杆菌属(Bacillus)为优势菌属。【结果】黑龙江省大豆胞囊线虫胞囊中存在丰富的细菌物种多样性,这些细菌对大豆胞囊线虫可能具有一定的生理生态作用。
[ Objective] To understand the bacterial diversity isolated from the cysts of Heterodera glycines in the soybean field in Heilongjiang Province. [ Methods] Bacteria were isolated from cysts on nutrient agar plates using dilution plate method and further identified by phylogenetic analysis based on 16S rDNA gene sequences. [ Results] Totally 90 bacteria strains with different colony morphology were selected on nutrient agar plate and their phylogenetic features were analyzed based on the partial 16S rDNA sequences. In total 7 genera and 22 species were identified, including 46 strains in Gammaproteobacteria （ 51. 1% ） , 32 in Firmicutes （ 35.6% ） , 10 in Betaproteobacteria （ 11.1% ） , and 2 in Alphaproteobacteria （2.2%）. The dominant bacteria species were Pseudomonas and Bacillus. [ Conclusion] There was abundant species diversity of bacteria isolated from cysts Heterodera glycines in Heilongjiang, and these bacteria may play a physical and ecological roles in nematodes.
出处
《微生物学报》
CAS
CSCD
北大核心
2012年第7期902-909,共8页
Acta Microbiologica Sinica
基金
公益性行业(农业)科研专项(20090304)
中国博士后科学基金(20100480028)~~
关键词
大豆胞囊线虫
胞囊
可培养细菌
多样性
Heterodera glycines, Cyst, Culturable bacteria, Diversity
