摘要
[目的]通过观察莪蚕健胃方对胃癌前病变大鼠胃黏膜组织细胞增殖、凋亡及Bcl-2蛋白表达的影响,探讨莪蚕健胃方治疗胃癌前病变的作用机制。[方法]采用N-甲基-N-硝基-N-亚硝基胍(MNNG)配合饥饱失常法诱导造模。设空白对照组,模型对照组,莪蚕健胃方大、中、小剂量组,进行常规病理检测,采用免疫组化SP法、TUNEL法检测胃黏膜组织细胞增殖核抗原(PCNA)、Bcl-2蛋白及凋亡指数(AI)的表达情况。[结果]莪蚕健胃方各剂量组胃黏膜组织病理学变化,中、重度异型增生率与模型对照组相比明显降低(均P<0.05);莪蚕健胃方能明显下调胃癌前病变组织细胞的PCNA、Bcl-2的表达,上调AI。[结论]莪蚕健胃方对大鼠实验性胃黏膜癌前病变有逆转作用,抑制细胞增殖、诱导细胞凋亡可能是该方的作用机制之一。
[Objective]To investigate the effect of Ecan Jianwei Recipe on the proliferation and apoptosis of mucosa cells in the gastric precancerous lesions of rats. [Methods]The model was es- tablished with N-methyl-N-nitro-N-nitrosoguanidine（MNNG）and irregular diet. All rats were di- vided randomly into blank control group, model group, high-dosage Ecan Jianwei Recipe group, middle-dosage Ecan Jianwei Recipe group and low-dosage Ecan Jianwei Recipe group. Routine pa- thology was examined. The expression of PCNA and Bcl-2 protein and apoptosis index（AI）was detected by immunohistochemistry and TUNEL respectively. [Results] Compared with model group,three Ecan Jianwei Recipe groups had significant difference in histopathological changes of gastric mucosa（P〈0.05）. Ecan Jianwei Recipe can down-regulated the expression of PCNA and Bcl-2 protein and up-regulated AI. [Conclusion]Ecan Jianwei Recipe has reversing effect on gastric precancerous lesions. Inhabiting cell proliferation and inducing cell apoptosis may be one of mechanisms of Ecan Jianwei Recipe treating gastric precancerous lesion.
出处
《中国中西医结合消化杂志》
CAS
2012年第6期256-259,共4页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
关键词
胃癌前病变
莪蚕健胃方
增殖
凋亡
gastric precancerous lesions
Ecan jianwei recipe
proliferation
apoptosis
