摘要
[目的]探讨重症急性胰腺炎(SAP)时肺组织Ⅱ型分泌型磷脂酶A2(sPLA2-Ⅱ)的表达及功能改变。[方法]将SD大鼠随机分为假手术组(SO组,n=10)、模型组(SAP组,n=10)。SO组仅行剖腹术,翻动胰腺;SAP组用去氧胆酸钠胰管逆行注射建立SAP合并肺损伤模型。2组动物在术后24h测pH、PaO2、PaCO2、血淀粉酶、sPLA2-Ⅱ,肺湿/干比值。应用RT-PCR、western-blot观察肺组织sPLA2-Ⅱ表达,并观察胰、肺组织病理变化。[结果]SAP组血淀粉酶、sPLA2、肺湿/干比值显著高于SO组(P<0.05)。SAP组PaO2、pH显著低于SO组(P<0.05),PaCO2、sPLA2-Ⅱ显著高于SO组(P<0.05)。[结论]SAP时肺组织sPLA2-Ⅱ表达增高,可能是急性肺损伤的发病机制之一。
Abstract： [Objective]To investigate the expression of secretory type II phospholipase A2 （sPLA2-Ⅱ ）in lung in rats with severe acute pancreatitis. [Methods]SD rats were randomly divided into 2 groups of sham operation group（SO,n： 10）and SAP model group（SAP, n= 10）. Severe acute pancreatitis was induced in SAP. Sham operation was only made in SO group. Serum amyl- ase（AMY） levels, Ph, PaO2, PaCO~, sPLA2 and lung wet/dry ratio （W/D） were determined, sPLA2- [[ mRNA expression in lung was detected by RT-PCR. The sPLA2- [[ protein expression in lung was detected by western-blot. The pathologic changes of pancreas and lung were observed 24 hours after establishment of the model. [Results]Serum levels of AMY（J7144.19±727. 91]U/ L） ,sPLA2 （[45.13±6.05]nmol.min^-1. ml^-1） ,W/D（8. 57±2.45）and PaCO2 （[47.57±2.55] mmHg）in SAP were remarkably higher than those in SO（[1193.41±192.54]U/L, [-29. 94± 6. 39]nmol. min^-1 .ml^-1, [3.70±0.90], [27. 69±1.02]mmHg, P〈0.05）. The levels of PaO2（[-79.24± 5. 841 mmHg）and pH（7. 269±0. 054）in SAP were lower than that in SO（[-96.78± 3. 811 mmHg,7. 391±0. 054,P〈0.05）. The expression of sPLA2-Ⅱ in lung in SAP was signifi- cantly increased than in SO（P〈0.05）. The pathologic changes of pancreas and lung in SO group were milder than that in SAP group. [Condusion]sPLA2- Ⅱ increased remarkably in acute lung injury（ALI）induced by SAP, which might be important in ALl induced by SAP.
出处
《中国中西医结合消化杂志》
CAS
2012年第6期241-244,共4页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
基金
国家自然科学基金资助项目(No:30973851)
