摘要
目的 :探讨肺炎患儿肺炎支原体 (MP)感染的实验室检测方法。方法 :使用聚合酶链反应(PCR)技术检测 676例肺炎患儿MP的感染 ,其中 1 3 9例同时采用支原体培养进行检测 ,1 1 2例做间接血凝试验 (IHA)。结果 :PCR法对MP感染的检出率明显高于培养法 (P <0 .0 1 ) ,双份血清IHA的检出率和PCR法比较无显著性差异 (P >0 .0 5 ) ,单份血清IHA的检出率明显低于PCR法(P <0 .0 1 )。结论 :PCR法是检测MP感染的简便有效的方法。PCR和IHA方法结合使用 ,可提高MP感染的检出率。
Objective:To evaluate the methods for detection and diagnosis of mycoplasma infections in children with pneumonia.Methods:The polymerase chain reaction (PCR) technique,mycoplasma culture and indirect haemagglutination assay (IHA) were used to detect mycoplasma pneumoniae in clinical samples obtained from 676 children (411 male and 265 female) with pneumonia.Results:Of 676 patients with pneumoniae,115 children were confirmed to be positive of mycoplasma pneumoniae.The positive rate of detection was significantly higher with PCR than with mycoplasma culture ( P < 0.01 ).There was no significant difference between the positive rate of detection with PCR and that with IHA ( P >0.05).Conclusion:PCR method and IHA are highly applicable in routine clinical laboratories for the preliminary detection and diagnosis of mycoplasmal infections,particularly in extrapulmonary cases.
出处
《浙江大学学报（医学版）》
CAS
CSCD
2000年第2期67-69,共3页
Journal of Zhejiang University(Medical Sciences)
关键词
肺炎支原体
肺炎
聚合酶链反应
儿童
Pneumoniae,mycoplasma/diag
Pneumoniae,mycoplasma/blood
Polymerase chain reaction
Mycoplasma,pneumoniae/isol
Children
