摘要
目的:评价新藤黄酸对S180肿瘤细胞株的体内外抗肿瘤作用。方法:将不同浓度的新藤黄酸分别作用于体外培养的S180细胞48 h,用CCK-8法检测药物对S180细胞的增殖抑制作用;以8.0,4.0,2.0 mg·kg-1剂量ip给予S180腹水瘤小鼠,每天1次,连续7 d,观察45 d对存活时间的影响;以16.0,8.0,4.0 mg·kg-1剂量ig给予荷S180实体瘤小鼠,每天1次,连续12 d,评价新藤黄酸的体内抗肿瘤作用;测单次ip给药对小鼠的LD50。结果:新藤黄酸对体外培养S180细胞的增殖有明显的抑制作用,药物作用48 h其IC50为(1.54±0.12)mg·L-1;4.0 mg·kg-1新藤黄酸ip给药可使S180腹水瘤小鼠存活时间较荷瘤对照组延长141.6%,新藤黄酸对S180实体瘤的抑制作用随着剂量的增大而升高,并呈一定剂量依赖性,体内抗肿瘤显著(P<0.05);单次ip给药对小鼠的LD50为36.66 mg·kg-1。结论:新藤黄酸对S180细胞株具有明显的体内外抗肿瘤作用。
Objective： To evaluate the anti-tumor effects of neogambogic acid on S180 cell line both in vivo and in vitro. Method： CCK-8 method was used to assay the anti-proliferative effect of neogambogic acid with different concentrations for 48 h on S180 cell line in vitro. The anti-tumor effect in vivo was evaluated by the survival time of the mice bearing S180 ascitic tumor, which was ip treated with 8.0, 4. 0, 2.0 mg.kg^-1 neogambogic acid respectively, once per day for continuous 7 days, and observation period lasted for 45 days, and the mice bearing S180 xenograft was ig treated with 16.0, 8.0, 4.0 mg.kg^-1 neogambogic acid respectively, once per day for continuous 12 days. The LD50of mice was determined based on single administration （ip） of different doses of neogambogic acid. Result： Neogambogic acid showed obvious anti-proliferative effect on S180 cell culture, the IC50 of 48 h was （ 1.54 ± 0. 12） mg. L^-1 And the in vivo test showed that the survival time of the mice administrated with 4.0 mg. kg^-1 neogambogic acid was prolonged 141.6%. The inhibitory effects of the mice bearing tumor increased with the increasing of the concentration of neogambogic acid in a dose-dependent manner （P 〈 0.05）. The LD50f neogambogic acid （ip） was 36.66 mg. kg^-1. Conclusion ： The results suggest that theanti-tumor effect of neogambogic acid on S180 cell line is significant both in vivo and in vitro.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第13期193-197,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东省高等学校科技创新团队项目(06CXTD004)
广东省科技计划重点项目(2008A030101002)
东莞市科技计划高等院校和科研机构项目(2007108101080)
