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腺病毒载体介导IL-24基因对肺癌细胞的化疗增敏效应 被引量：4

Recombinant adenovirus expressing IL-24 enhances the sensitivity of lung cancer cell to chemotherapeutic drugs
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摘要 目的研究携带人IL-24基因腺病毒载体(Ad-IL-24)联用顺铂(DDP)对人非小细胞肺癌NCI-H460细胞生长和凋亡的影响。方法取对数生长期的NCI-H460细胞,MTT比色法检测Ad-IL-24联合DDP对细胞生长的影响,流式细胞仪分析细胞周期及检测细胞凋亡率。结果 Ad-IL-24联合DDP对NCI-H460细胞的生长抑制作用和诱导凋亡效应均明显优于单用Ad-IL-24和单用化疗药物DDP,且G2/M期细胞比例显著增加(P<0.05或<0.01)。结论 Ad-IL-24能提高NCI-H460肺癌细胞对化疗药物DDP的敏感性。 Objective To investigate the effects of recombinant adenovirus expressing IL-24 （Ad-IL- 24） combined with cisplatin（DDP） on growth and apoptosis of human non-small cell lung cancer NCI-H460 cells. Methods NCI-H460 cells in logarithmic phase were prepared. Effects of Ad-IL-24 combined with DDP on cell growth of NCI-H460 cells were detected by MTI＂ assay. Cell cycle and apoptosis were analyzed by flow cytometry. Results Compared with any single group, the inhibition of cell gro^h and cell apoptosis in com- bined group are more effective. Furthermore, Ad-IL-24 combined with DDP significantly increased the ratio of cells in G2/M phase（P 〈0.05 or 〈0.01）. Conclusion Recombinant adenovirus expressing IL-24 could en- hance the sensitivity of lung cancer cells to chemotherapeutic drugs DDP.
作者 李畅 赵军 李印 梁光辉 盛伟华 杨吉成
机构地区 苏州大学附属第一医院心胸外科 河南省肿瘤医院胸外科 苏州大学医学部基础医学与生物科学学院细胞与分子生物学教研室
出处 《苏州大学学报（医学版）》 CAS 2012年第3期322-325,共4页 Suzhou University Journal of Medical Science
基金 江苏省"六大人才高峰"第八批资助项目计划
关键词 腺病毒 人白细胞介素-24 肺癌 基因治疗 顺铂 adenovirus IL-24 pulmonary carcinoma gene therapy cisplatin
分类号 R734.2 [医药卫生—肿瘤]
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参考文献16

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共引文献117

同被引文献43

  • 1韩宝惠.肺癌治疗中若干热点问题的探讨[J].临床肿瘤学杂志,2007,12(10):721-727. 被引量：11
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  • 7Jiang G, Zhang K, Jiang AJ, et al. Aconditionally replicating adenovirus carrying interleukin-24 sensitizes melanoma cells to radiotherapy via apoptosis[J]. Mol Oncol, 2012, 6(4): 383 -391.
  • 8lin BW, Jiao ZL, Fan JF, et al.lnhibitory effect of melanoma differentiation associated gene-7/interleukin-24 on invasion in vitro of human melanoma cancer cells [J]. Korean Med Sci, 2013, 28 ( 6 ) : 833 - 839.
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苏州大学学报（医学版）
2012年 第3期

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