摘要
酰胺酶是一种合成手性羧酸和酰胺衍生物的重要工具酶,在不对称合成中具有巨大应用潜力。传统的色谱分析方法通过检测底物及相应的产物去测定酰胺酶活性及立体选择性,十分费时费力。根据显色法、荧光法、NMR等原理,较为全面地综述了近年来国内外发展起来的高通量酰胺酶筛选方法。为酰胺酶筛选中筛选效率低、可靠性差等难题提供借鉴,也可为其他水解酶筛选模型的建立提供思路。
Being an important tool enzyme for the synthesis of chiral carboxylic acid and amide derivatives amidasc has great application potential in asymmetric synthesis. Activity and stercosclectivity of the amidase was usually as- sayed by traditional chromatographic analysis of substrates and the corresponding reaction products. It is very time- and-effort-consuming. A roundup of high throughput amidase screening methods developed in recent years both home and abroad according to the principles of eolorimetry, fluorimctry, and nuclear magnetic resonance （NMR） is summarized comprehensively in this paper to provide references for solving problems such as low efficiency and poor reliability in screening amidase, also to provide ideas for the establishment of screening model for other hydrolases.
出处
《微生物学杂志》
CAS
CSCD
2012年第3期66-71,共6页
Journal of Microbiology
基金
国家重点基础发展计划("973"计划:2011CB710806)
关键词
酰胺酶
高通量筛选
酶活检测
立体选择性
amidase
high-throughput screening
enzyme assay
stereocelectivity
