摘要
从引进Viili乳制品中筛选、鉴定出3种优良乳酸菌。采用平板分离法从Viili乳制品中分离3株乳酸菌,通过表型1、6S rRNA的PCR扩增、克隆、测序鉴定。3株乳酸菌的表型鉴定结果符合伯杰细菌鉴定手册中乳杆菌属的干酪乳杆菌,16S rRNA序列同源性分析结果表明3株分离菌与干酪乳杆菌的同源性分别为99.93%、100.00%9、9.78%,从Viili乳制品分离到3株干酪乳杆菌。
Three fine lactic acid bacteria strains from imported Viili dairy were screened and isolated adopting plate i- solation method by means of their phenotype, 16S rRNA PCR amplification, cloning, sequencing and other methods. The resuhs showed that their phenotypic identification results were in line with the LactobaciUus casei of the genus de- scribed in Bergey＇s Manual of Determination Bacteriology, 16S rRNA gene sequence homology analysis showed that the homologies of the three isolated strains with LactobaciUus casei were respectively 99.93% , 100.00% , 99.78%. Therefore, all of the three strains isolated from Viili dairy products were Lactobacillus casei.
出处
《微生物学杂志》
CAS
CSCD
2012年第3期47-52,共6页
Journal of Microbiology
基金
云南省重点基金(2011FA014)
云南省自然科学基金(2009CD058)
云南省高端科技人才引进计划项目(2009CI125)
云南省高校科技创新团队(云教科2011-14号)
关键词
干酪乳杆菌
分离
鉴定
16S
RRNA
lactic acid bacteria
separation
identification
16S rRNA
