摘要
目的分析登革病毒的基因组序列,了解目前世界范围存在的各种登革病毒基因型,探索部分中国毒株的可能来源。方法分析GenBank数据库中有完整基因组序列的登革病毒序列,利用NCBI服务器对基因组编码区序列进行在线比对,按最小进化法用MEGA5.0软件绘制种系发生树。结果分析了全球范围四种血清型共3000余株登革病毒的基因组序列,分别将各血清型毒株划分为4-6个基因型。通过对各基因型毒株背景的分析,证明基因型分布与毒株的地理来源存在一定联系。39株中国毒株中,多数能够通过种系发生树确定其来源。结论全球登革病毒的基因型分布具有地区特异性,中国登革毒株的传播来源存在多样性。
Dengue is the most wide-spread arthropod-borne viral disease of humans in the tropic and sub-tropic regions.In this study,genomic sequences of more than 3 000 dengue viruses available in the GenBank were aligned and analyzed by serotype.According to phylogenetic trees generated by the minimum evolution method of MEGA5.0,dengue viruses were divided into 4-6 genotypes within the four serotypes,respectively.Meanwhile,it was indicated that the distribution of most genotypes was associated with geographic origins of dengue viruses.Probable origins for most of the 39 strains from China with genomic sequences were deduced from relevant ancestral strains in the context of ME trees.These results revealed that the genotype distribution of dengue viruses was geographic origin-specific at genomic level,and that diverse introduction sources were attributed to dengue outbreaks in China.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2012年第6期515-521,共7页
Chinese Journal of Zoonoses
基金
supported by Natural Science Foundation of Fujian Province(No.2011J01126)~~
关键词
登革病毒
基因组
序列分析
dengue virus
genome
sequence analysis
