摘要
人肠道病毒及胃肠炎病毒借助水体传播可能引起水源影响区域的疫情暴发。尤其对免疫力发育尚不完全的儿童和免疫缺陷人群,这些病毒感染可能会引起严重的临床症状和并发症。因此,做好水源中肠道病毒及胃肠炎病毒的检测和监测,将对这些病毒所致疾病的防范和控制有重要意义。本文综述了目前国内外针对水体中的病毒浓缩和检测所做的相关研究,包括从水样中浓缩、纯化病毒的不同方法及建立在分子生物学和细胞培养基础上的病原检测方法。这些研究为进一步建立行业标准和实验室检测规范提供了重要的参考和技术依据。
Enteroviruses and gastroenteritis viruses in water may lead to disease outbreaks around the contaminated area. The infections can result in severe clinical symptoms and complications in an immuno- compromised population as well as in children, whose immunity is not fu/ly developed. Therefore, effective detection and monitoring of enteroviruses and gastroenteritis viruses from water sources is critical in the prevention and control of associated diseases caused by their infection. The current progresses on virus enrichment methods and molecular- or cell-based virus detection methods for water samples are reviewed in this paper. A better understanding of these methods will help us to build the standards for industry and laboratory standards.
出处
《微生物与感染》
2012年第2期111-116,共6页
Journal of Microbes and Infections
基金
上海市卫生局资助项目(研2011-16)
关键词
滤膜浓缩法
聚乙二醇沉淀法
蚀斑试验
Membrane adsorption-elution method
Polyethylene glycol precipitation method
Plaque assay
