摘要
目的在模拟人体生理条件下,研究金属离子对五味子甲素与牛血清白蛋白(BSA)之间结合作用的影响。方法采用紫外吸收光谱法,通过紫外扫描得到五味子甲素与BSA的紫外吸收图谱,利用Lineweaver-Burk双倒数曲线图得出五味子甲素与BSA的结合常数;由摩尔比法确定Mg2+等7种金属离子与五味子甲素结合的摩尔比;分别测定五味子甲素-金属离子-BSA的结合常数。结果五味子甲素与BSA的结合常数为1.141×104L/mol,金属离子与五味子甲素结合的摩尔比为1∶1,在Fe3+、Zn2+、Al3+、Cu2+、Mg2+、Co2+、Ni2+存在下,五味子甲素与BSA的结合常数分别为3.041×104,1.617×104,1.819×104,1.781×104,1.277×103,7.107×103,6.238×103L/mol。结论 Fe3+、Zn2+、Al3+、Cu2+参与加强了五味子甲素与BSA的结合作用,Mg2+、Co2+、Ni2+的存在使二者结合作用减弱。
Objective To study the effect of metalion on the interaction between Deoxyschizandrin and bovine serum albumin （BSA） under the human physiological condition. Methods The UV absorption spectra d Deoxyschizandrin and BSA was obtained by ultraviolet absorption spectroscopy. The binding constant of Deoxyschizandrin -BSA was measured using the Lineweaver-Bttrk double reciprocal diagram of curve.The proportions of seven metalions binding to Deoxysehizandrin were determined by the method of mole ratio. The binding constants Deoxysehizandrin -BSA were obtained separately. Results The bhading constant of Deoxysehizandrin -BSA was K=I. 141 ~ 104 L/tool. All the mole ratios of Deoxysehizandrin -Men＋ was 1 ： 1 and the binding constants of Deoxysehizandrin -BSA in presence of seven metal ions were 3.041 × 10^4, 1.617 × 10^4, 1.819 ×10^4, 1.781 × 10^4, 1.277 ×10^3, 7.107 ×10^3, 6.238 × 10^3 ldmol.Condusion In presenee of Fe3＋, Zn2＋, A13＋ or Cu2＋, the interaction between Deoxysehizandrin and BSA has been strengthened, which has been weakened in presence of other ions.
出处
《基层医学论坛》
2012年第16期2052-2055,共4页
The Medical Forum
关键词
五味子甲素牛血清白蛋白
紫外吸收光谱法金属离子
Deoxyschizandrin Bovine serum albumin Ultraviolet absorption spectroscopy Metalion
