摘要
在23～25℃下,给平均体质量104.2g和106.8g的回交鲤(鲤鲫杂交♀×鲤♂)和回交荷包红鲤(鲤鲫杂交♀×荷包红鲤♂)胸腔注射植物凝集素(PHA)和秋水仙素,然后观察对比了染色体核型,探讨回交后代的染色体遗传。结果表明,两种鲤鲫杂交回交子代染色体组均由150条染色体组成,按着丝粒位置染色体组型可分为四组,每个染色体小组均由三条同源染色体组成。其中,回交鲤染色体数目为3n=150,染色体臂数NF=234,其核型公式为:3n=60m+24sm+36st+30t;回交荷包红鲤的染色体数目为3n=150,染色体臂数NF=252,其核型公式为:3n=66m+36sm+18st+30t。
The chromosome numbers and karyotypes were observed, and compared in offsprings of backcross of purse red carp （hybrids of common carp Cyprinus carpio and crucian carp ♂ Carassius auratus × purse red carp 8 Cyprinus carpio var. wuyuanensis） by intraperitoneal injection of phytohemagglutinin （PHA） and colchicines to evaluate the chromosome genetics of the offsprings. The results showed that the chromosome numbers of the two backcross offsprings of common carp and crucian carp were both 150, and divided into four chromosome groups of each being consisted of 3 homologous chromosomes, depending upon the position of centromere. In the backcross offsprings of the purse red carp, chromosome numbers and karyotypic formula were expressed to be 3n = 150, NF=252 and 3n=66m＋36sm＋18st＋30t. The backcross offsprings of common carp, chromosome numbers and karyotypic formula were found to be 3n=150, NF=234 and 3n =60m＋ 24sm＋ 36st＋30t.
出处
《水产学杂志》
CAS
2012年第2期7-10,14,共5页
Chinese Journal of Fisheries
基金
国家863计划项目(2011AA100404)
关键词
回交
荷包红鲤
鲤
染色体核型
backcross
Purse Red Carp
Common Carp
chromosome karyotype
