摘要
目的研究S-TK1含量在乳腺癌、乳腺良性肿瘤患者及健康体检人群中的差别;探讨S-TK1与乳腺癌的关系,探讨乳腺癌患者S-TK1的含量与肿瘤分期、肿块大小、组织学分级、ER、PR及HER-2之间的关系;进而分析S-TK1含量与乳腺癌患者复发、转移的关系。方法采用化学发光点印迹法,分析乳腺癌、乳腺良性肿瘤患者及健康体检者S-TK1的含量。结果 (1)乳腺癌患者治疗前(A组)、乳腺良性肿瘤患者(B组)及健康体检者(C组)S-TK1的含量分别为(17.98±10.20)pmol/L,(1.50±1.02)pmol/L,(0.88±0.45)pmol/L。A组S-TK1的含量明显高于B组和C组,差异有统计学意义(P均<0.001);B组S-TK1的含量高于C组,差异有统计学意义(P=0.017)。A组Ⅲ期乳腺癌患者S-TK1的含量(21.54±11.85)pmol/L高于Ⅱ期患者(14.05±6.19)pmol/L,差异有统计学意义(P=0.018)。(2)S-TK1的含量与组织学分级、HER-2及肿块大小均呈正相关(其相关系数分别为r=0.596、0.324、0.397,对应的P值为P<0.001、=0.042、=0.011);而ER与S-TK1的含量呈负相关(其r=-0.391,P=0.013);PR与S-TK1的含量无相关性(P=0.395,r=-0.138)。(3)复发转移组治疗前S-TK1含量(23.17±13.15)pmol/L明显高于无病生存组(14.31±5.50)pmol/L,差异有统计学意义(P=0.011),S-TK1低值组和中值组无病生存高于S-TK1高值组,但差异无统计学意义(P=0.186)。结论 S-TK1也许可以作为乳腺癌早期诊断的指标之一;S-TK1含量与乳腺癌患者的预后有关,治疗前高S-TK1含量可能更容易早期出现复发、转移。
Objective To study the difference of S-TK1 level among patients with breast cancer,patients with benign breast tumor and healthy volunteers,to investigate the correlation between S-TK1 level and staging,the size of tumor,grade,ER,PR or HER-2 in patients with breast cancer and analyze the mechanism that S-TK1 involved into the recurrence and metastasis of breast cancer patients.Methods The S-TK1 levels of breast cancer,breast benign tumor and healthy volunteers were detected by enhanced chemiluminescence（ECL） dot blot assay.Results（1）The concentrations of S-TK1 in patients with breast cancer before treatment（group A）,benign breast tumor（group B）（17.98±10.20）pmol/L,and healthy volunteers（group C）（1.50±1.02）pmol/L and（0.88±0.45）pmol/L,respectively.There was significant difference between group A and group B or group C（P0.001,P0.001）,and between group B and group C（P=0.017）.Also in group A the concentration of S-TK1 of staging Ⅲ was significantly higher than that of staging Ⅱ,（21.54±11.85）pmol/L vs.=（14.05±6.19）pmol/L（P=0.018）.（2）There was a positive correlation between the concentration of S-TK1 with grade,HER-2 and the size of tumor（r=0.596,0.324,0.397,P0.001,P=0.042,P=0.011,respectively） and a negative correlation between ER and the concentration of S-TK1（r=-0.391,P=0.013）.But the concentration of S-TK1 had correlation with PR（P=0.395,r=-0.138）;（3）The S-TK1 level in patients with recurrence and metastasis before therapy was higher than that in disease-free patients,（23.17±13.15）pmol/L vs.（14.31±5.50）pmol/L（P=0.011）.The DFS in low and middle S-TK1 level was longer than that in high S-TK1 level,but there was not significant diference（P=0.138）.Conclusion S-TK1 perhaps could be one of markers for early breast cancer diagnosis.The level of S-TK1 has relation with the progress of breast cancer,and results into recurrence and metastasis.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2012年第6期637-641,共5页
Cancer Research on Prevention and Treatment
关键词
胸苷激酶-1
乳腺癌
化学发光点印迹
Thymidine kinase-1
Breast cancer
Enhanced chemiluminescence dot blot assay
