摘要
本文利用生物素标记探针从细胞定位水平对50例原发性肝癌(HCC)手术活检标本进行了HBV DNA检测。 32例(64％)HCC切片中检出HBV DNA,其中25例(50％)系肝癌细胞与癌旁肝细胞内均检出HBV DNA,其余7例则仅在癌旁肝细胞内检出HBV DNA。光镜下观察,HBV DNA的分布具有胞浆内为主;癌旁肝细胞优势性、HCC阳性者同时伴有癌旁肝细胞阳性及不均一性等特点。上述结果提示至少占半数的HCC病例其癌细胞可能来自HBV DNA阳性肝细胞的恶变,但HBV DNA在恶变过程中的确切作用尚需进一步研究。
The paraffin embedded tissue sections from 50 patients with hepatocellular carcinoma (HCC) were examined for HBV DNA by hybridization in situ using a biotin-labelled probe. Thirty two cases (64.0％) were positive for HBV DNA, and in 25 cases the HBV DNA was found localized both in the para-tumorous hepatocytes and HCC cells, and in 7 cases only in para-tumorous hypatocytes. Patterns of HBV DNA distribution were characterized by predilection in cytoplasm, in para-tumorous hypatocytes, occurrence in both the HCC cells and para-tumorous hepatocytes and heterogeneity. The findings revealed that in more than half of the patients the HCC cell could be originated from malignant transformation of hepatocytes infected by HBV. However, the exact action of HBV DNA in the process of carcinogenesis needs to be elucidated.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
1990年第6期330-333,共4页
Chinese Journal of Clinical Oncology
关键词
肝癌
乙肝病毒
原位杂交技术
Hepatitis B virus (HBV)
Hepatocellular carcinoma
Hybridization in situ
