摘要
[目的]优化苎麻SSR反应体系。[方法]以苎麻湘苎2号基因组DNA为模板,对其SSR反应体系中的部分影响因素进行探讨分析,并利用选定的7个苎麻品种基因组DNA为模板,对优化的SSR反应体系进行试验验证。[结果]在25μl SSR反应体系中,模板量为50 ng,Mg2+浓度为1.5 mmol/L,dNTPs浓度为0.10 mmol/L,引物浓度为0.2μmol/L,Taq DNA聚合酶用量为1 U时,反应体系为最佳。利用该反应体系,对7个苎麻品种进行的SSR反应结果显示不同品种间DNA谱带多态性丰富,同时表明优化的反应体系重复性和稳定性良好。[结论]该研究结果为应用SSR技术进行苎麻种质资源的研究奠定良好的基础。
[ Objective] To optimize the reaction system of B. nivea. [ Method] With the genomic DNA of Xiangzhu No. 2 as the template,the in- fluencing factors of its SSR reaction system were analyzed,and then the optimized SSR reaction＇system was experimentally verified with the ge- nomic DNA of seven B. nivea varieties as template. [ Result ] The 25 μl SSR reaction system containing 50 ng DNA template, 1.5 mmol/L MgCl2, 0. 10 mmol/L dNTPs,0.2 μmol/L primer,and 1 U Taq DNA polymerase was the best. By using the reaction system,the SSR reaction results of seven B. nivea cuhivars showed abundant polymorphism between different cultivars, and the optimized reaction system had good repeatability and stability. [ Conclusion] The experimental results lay foundation for the study of B. nivea resources by SSR technology.
出处
《安徽农业科学》
CAS
2012年第17期9206-9208,共3页
Journal of Anhui Agricultural Sciences
基金
湖南省科技厅重点项目(2010TP4004-1
2010FJ2013)
关键词
苎麻
SSR
反应体系
优化
Boehmeria nivea （ L. ） Gaud
SSR
Reaction system
Optimization
