摘要
目的:研究NaCl刺激对保加利亚乳杆菌ATCC11842生长的影响,分析NaCl刺激下此菌细胞膜流动性和质膜脂肪酸组成的变化。方法:经不同浓度NaCl处理后,用荧光探针1,6-二苯基-1,3,5,-己三烯(DPH)研究细胞膜流动性的改变;用气相色谱法测定NaCl刺激下细胞膜脂肪酸组成的改变。结果:保加利亚乳杆菌能耐受的最高NaCl浓度为1.0mol/L。确定了在保加利亚乳杆菌中DPH孵育时间(60min)和温度(35℃)条件。当NaCl浓度为0.4mol/L时,此菌株中主要的脂肪酸为C16:0和C18:1;随着NaCl浓度增加,饱和脂肪酸/不饱和脂肪酸比率(SFA/UFA)从原来的0.57增加到0.78;同时,保加利亚乳杆菌存活率逐渐降低。结论:当NaCl浓度从0.2mol/L增加到0.8mol/L时,随着荧光偏振值和微黏度的增加,细胞质膜流动性减小,并且膜中饱和脂肪酸比率增加。
Objective：Effect of NaCl stimulation on the growth,membrane fluidity and membrane fatty acid composition of Lactobacillus bulgaricus ATCC11842 were investigated.Methods：Fluorescent probe（1,6-diphenyl-1,3,5-hexatirene,DPH）were used to analyze the fluidity（membrane polarization）of the cellular cytoplasmic membrane after stimulation of different concentrations of NaCl levels.Meanwhile,Gas chromatography（GC）was used to analyze fatty acid composition of cytoplasmic membrane under the same conditions.Results：The maiximum tolerance of L.bulgaricus was up to 1.0mol/L NaCl.Major fatty acids of this bacterium were palmitic acid（16：0）and cis-vaccenic acid（18：1）under salt stimulation（0.4mol/L NaCl）.The salt concentration strongly influenced the fatty acid composition.the rate of saturation to unsaturation increased from 0.57 to 0.78 as the increasing of NaCl concentrations.Meanwhile,the growth of L.bulgaricus decreased.Conclution：When the concentration of NaCl was increased from 0.2mol/L to 0.8mol/L,the bacterial cytoplasmic membrane fluidity decreased with the fluorescence polarization increasing and the ratio of the fatty acid in cytoplasmic membrane increased.
出处
《食品工业科技》
CAS
CSCD
北大核心
2012年第12期132-135,139,共5页
Science and Technology of Food Industry
基金
国家教育部长江学者和创新团队发展计划(IRT0959)
东北农业大学博士启动基金(2010RCB59)
关键词
保加利亚乳杆菌
盐刺激
荧光探针
细胞膜流动性
脂肪酸
Lactobacillus bulgaricus； salt stimulation； fluorescent probe； membrane fluidity； fatty acid
