摘要
目的观察人β干扰素（IFN—β）基因转染的人骨髓间充质干细胞（hMSCs）对前列腺癌细胞株PC一3增殖及捌亡的影响。方法脂质体介导入IFN—p基因转染的hMSCs（IFN—β—hMSCs）与PC-3体外共培养，共培养后第1、3、5天对PC-3进行锥虫蓝染色，计数存活细胞数目。流式细胞仪分析PC．3细胞凋亡。结果IFN-β—hMSCs可成功分泌表达IFN—β。hMSCs、空质粒转染hMSCs、IFN—β—hMSCs组与PC-5在共培养后第5天，PC-5存活细胞计数分别为（10．81±0．33）×10^5、（9．90±0．41）×10^5、（4．63±0．32）×10^5个，PC-3存活细胞差异有统计学意义（P〈0．01）；流式细胞仪检测示实验组PC-3凋亡率为（22．79±2．71）％，较各对照组增加。结论IFN—β—hMSCs能成功分泌表达IFN—β，体外共培养可有效抑制PC-3增殖，促进其凋亡。
Objective To investigate the effect of human interferon -β （IFN-β） gene cDNA engineered human bone marrow mesenchymal stem cells （hMSCs） on the proliferation and apoptosis of prostate cancer cell line PC-3. Methods The pCDNA3.1-IFN-β vector containing human IFN-β gene was constmcted and transfected into hMSCs by lipofectamine. IFN-β-expressing mesenchymal stem ceils （IFN-β- hMSCs） were incubated with PC-3 cells. PC-3 cells were stained by trypan blue and viable cells were counted by hemocytometer respectively at the 1 st, 3rd, and 5th day after co-culture. The apoptosis of PC-3 cells was analyzed by flow cytometry. Results IFN-β-hMSCs could express IFN-β successfully. Five days after co-cuhure, the number of viable PC-3 cells in hMSCs, mock-hMSCs, IFN-β-hMSCs groups was （10. 81 ±0. 33） × 10^5 , （9. 90 ±0. 41） × 10^5, and （4. 63 ±0. 32） × 10^5 respectively. IFN-β-hMSCs resulted in a significant decrease in PC-3 cells survival as compared with control group （P 〈 0. 01 ） , and apoptosis rate of PC-3 cells was （22.79 ± 2. 71 ）% , which was increased as compared with control group. Conclusion The IFN-β-hMSCs can express IFN-β successfully and inhibit the growth of prostate cancer cells significantly.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2012年第6期1056-1058,共3页
Chinese Journal of Experimental Surgery
基金
基金项目：国家自然科学基金资助项目（30560153）
关键词
前列腺癌
Β干扰素
间充质干细胞
基因治疗
Prostate carcinoma
Interferon beta
Mesenchymal stem cell
Gene therapy
