摘要
目的研究中国南海侧扁软柳珊瑚Subergorgia suberosa的次级代谢产物。方法利用硅胶柱层析、Sephadex LH-20凝胶柱层析、HPLC等方法对化合物进行分离纯化;利用NMR,MS等波谱方法,并与文献对照,鉴定化合物的结构;利用抗菌活性模型和卤虫致死活性模型评价化合物的生物活性。结果从侧扁软柳珊瑚中分离鉴定了6个单体化合物,分别为:倍半萜化合物subergorgic acid(1),2-β-hydroxysubergorgic acid(2)和2-β-acetoxysubergorgic acid(3);甾体化合物3β-hydroxy-5α-pregnan-20-one(4),5α-pregnane-3,20-di-one(5)和胆甾醇(6)。结论化合物1～3对白色葡萄球菌Staphylococcus albus均显示出较强的抑制活性,化合物4对蜡状芽孢杆菌Bacillus cereus显示出较强的抑制活性,而化合物4和5显示出较强的卤虫致死活性。
Objective To investigate the secondary metabolites ot： gorgonian coral Subergorgia suberosa. Methods Column chromatography on silica gel and Sephadex LH-20, and semi-preparative HPLC were used to isolate and purify the secondary metabolites. The structures of isolated compounds were finally identified by spectroscopic analysis including NMR and MS, and by comparison with the data of litera- ture. The bioactivities of the compounds were evaluated with antibacterial and brine shrimp lethality models. Results Six compounds were isolated from S. suberosa, and their structures were determined as three sesquiterpenes subergorgic acid （1）, 2-β-hydroxysubergorgie acid （2） and 2-β-aeetoxysubergorgic acid （3）, and three steroids： 3β-hydroxy-5α-pregnan-20-one （4）, 5α-pregnane-3,20-dione （5） and cho- lesterol （6）. Conclusion Compounds 1~3 exhibited potent antibacterial activity against Staphylococcus albus, and 4 exhibited antibacterial activity against Bacillus cereus. Compounds 4 and 5 showed brine shrimp lethality toward Artemia salina.
出处
《中国海洋药物》
CAS
CSCD
北大核心
2012年第3期11-16,共6页
Chinese Journal of Marine Drugs
基金
国家自然科学基金(41176121)
山东省自然科学基金(ZR2011HM085
ZR2011DQ019)
国家教育部高等学校博士学科点专项科研基金(20090132110002)
关键词
柳珊瑚
次级代谢产物
倍半萜
甾体
gorgonian coral
secondary metabolites
sesquiterpene
steroid
