摘要
目的 探讨喷他脒对卵巢癌Skov-3细胞及其裸鼠移植瘤PRL-3的抑制作用.方法 （1）M TT法检测不同浓度喷他脒对Skov-3细胞的生长抑制作用;（2）RT-PCR法检测不同浓度喷他脒处理后的Skov-3细胞PRL-3的表达;（3）建立人卵巢癌Skov-3 细胞裸鼠转移瘤模型,按喷他脒给药剂量分4组,记录裸鼠皮下移植瘤的生长情况,绘制肿瘤生长曲线.采用RT-PCR方法检测裸鼠移植肿瘤的PRL-3表达情况.结果 喷他脒对卵巢癌Skov-3细胞体外生长有抑制作用,呈浓度依赖性（P＜0.05）;喷他脒中高剂量组予5次给药后裸鼠移植瘤生长速度均与对照组有差异（P＜0.05）;中、高剂量喷他脒抑制人Skov-3卵巢癌细胞裸鼠皮下移植瘤的PRL-3表达,且随剂量增加而抑制作用更明显（P＜0.05）.结论 喷他脒对卵巢癌Skov-3细胞体外生长及裸鼠Skov-3皮下移植瘤均有有抑制作用;喷他脒可抑制Skov-3卵巢癌细胞及裸鼠Skov-3皮下移植瘤PRL-3的表达.
Objective To investigate the inhibitory effect of pentamidine on cell growth and PRL-3 expression of ovarian cancer Skov-3 cell line in vitro and in vivo. Methods Human epithelial ovarian cancer Skov-3 cells were treated with different concentrations pentamidine in vitro; the cell growth was evaluated by MTT assay, the expression of PRL-3 was detected by RT-PCR. The Skov-3 cells were subcutaneously inoculated into nude mice to establish the animal ovarian cancer model. Thirty two tumor-bearing nude mice were randomly divided into 4 groups, and each group received different dose of pentamidine. Tu- mor growth was observed and tumor volume was continuously measured, then the growth inhibition rate was calculated. The PRL-3 expression in excised tumor was detected by RT-PCR. Results Pentamidine inhibited cell proliferation in vitro in a dose-dependent manner （P〈O.05）. The growth of xenograft tumor in nude mice treated with medium-and large-dosage of pen- tamidine was significantly slower than that in control group. The treatment of pentamidine at medium- and large-dosage signifi- cantly inhibited PRL-3 expression of xenograft tumor in a dose-dependent manner, with stronger effect in higher dose. Conclusion Pentamidine inhibits cell proliferation of human epithelial ovarian cancer Skov-3 cells in vitro and xenograft tumor growth in vivo. Pentamidine can also inhibit the expression of PRL-3 in Skov-3 cells in vitro and in vivo.
出处
《浙江医学》
CAS
2012年第9期678-680,I0001,共4页
Zhejiang Medical Journal
基金
浙江省医药卫生科学研究基金（2009B081）
浙江省自然科学基金（Y2090319）
南京军区医药卫生科研项目（09MA107）
