摘要
目的:将新兴的结核特异性IFN-γ酶联免疫斑点法(ELISPOT)应用于附睾结核检测,并比较其与传统的结核菌素皮试(PPD)在附睾结核及健康对照组中检测的差异,探讨ELISPOT在附睾结核诊断中的应用前景。方法:采用自主研制的ELISPOT试剂盒对13例附睾结核患者进行检测;采用PPD对11例附睾结核患者检测。同时采用2种方法对57例健康男性志愿者进行平行检测。结果:附睾结核患者,ELISPOT阳性率92.3%(12/13),PPD阳性率90.9%(10/11),2种方法差异无显著性(P>0.05)。健康志愿者中ELISPOT阳性率14.0%(8/57),PPD阳性率49.1%(28/57),两者差异有极显著性(P<0.01)。结论:附睾结核检测中ELISPOT在敏感性上与PPD一致,在区分附睾结核与健康人方面特异性优于PPD,在附睾结核诊断上有较好的应用前景。
Objective： To explore the potential application of IFN-γ enzyme-linked immunospot （ELISPOT） assay in the diag- nosis of tuberculous epididymitis （TE） by comparing ELISPOT assay with the traditional purified protein derivative （PPD） tuberculin skin test. Methods： We examined 13 TE patients using an in-house ELISPOT kit, another 11 TE patients by PPD skin testing, and 57 healthy male volunteers by parallel test with both the methods. Results： Twelve （92.3%） of the 13 TE cases were positive on ELISPOT assay, and 10 （90.9%） of the 11 TE cases positive on PPD skin test, with no statistically significant differences between the two groups （ P 〉 0.05 ）. Among the 57 healthy male volunteers, 8 （ 14.0% ） were positive on ELISPOT, and 28 （49.1% ） positive on PPD test, the latter significantly higher than the former （ P 〈0. 001 ）. Conclusion ： In terms of sensitivity, ELISPOT assay is similar to PPD test in the examination of tuberculous epididymitis. As for specificity, ELISPOT assay seems better than PPD test in differentia- ting tuberculous epididymitis patients from healthy males.
出处
《中华男科学杂志》
CAS
CSCD
2012年第6期534-537,共4页
National Journal of Andrology
基金
深圳市科技计划项目(201103133)~~
关键词
附睾结核
酶联免疫斑点法
结核菌素皮试
tuberculous epididymitis
enzyme-linked immunospot assay
PPD skin test
