摘要
目的:观察针刺对帕金森病模型小鼠行为学和纹状体神经元凋亡的影响,探讨针刺治疗帕金森病的机制。方法:成年C57/BL雄性小鼠28只,随机分为正常组、模型组、针刺组和西药组。腹腔注射1-甲基-4-苯基-1,2,3,6-四氢吡啶复制帕金森病模型。针刺组给予"百会""风府"针刺及"阳陵泉"电针治疗,每日1次,每次15min,治疗14d;西药组给予多巴丝肼灌胃治疗,每日1次,治疗14d。分别在造模前、造模后和治疗后对各组小鼠进行自主活动计数、爬杆实验、游泳实验的行为学检测,采用TUNEL法测定各组小鼠纹状体神经元凋亡指数。结果:模型组小鼠造模后自主活动计数、游泳实验评分显著低于正常组(P<0.01),爬杆实验评分显著高于正常组(P<0.01);针刺组、西药组小鼠治疗后自主活动计数、游泳实验评分显著高于模型组(P<0.05,P<0.01),爬杆实验评分显著低于模型组(P<0.05)。模型组、针刺组、西药组纹状体神经元凋亡指数均显著高于正常组(P<0.01),针刺组凋亡指数显著低于模型组(P<0.01)。结论:针刺"百会""风府""阳陵泉"可改善小鼠自主活动、爬杆、游泳等行为,减少纹状体神经元凋亡,改善帕金森病的运动症状。
Objective To observe the effect of acupuncture of ＂Baihui＂ （GV 20）, ＂Fengfu＂ （GV 16 ） and ＂Yanglingquan＂ （GB 34） on behavior and striatal apoptosis in Parkinson disease （PD） mice. Methods Twenty-eight adult male C57/ BL mice were randomly divided into normal control, PD model, acupuncture and medication （Levodopa and Benserazide Hydrochloride） groups. Acupuncture was applied to ＂Baihui＂（GV 20）, ＂Fengfu＂ （GV 16） and electroacupuncture （2 Hz, 1 mA） was ap- plied to ＂Yanglingquan＂（GB 34） for 15 min, once a day for 14 days. PD model was duplicated by intragastric administration of 1- methyl-4-phenyl-1,2,3,6-tetrahydropyridine （MPTP）. The locomotor test, pole test and swimming test were conducted for assessing the animals＇ locomotion behavior before and after MPTP administration and after the treatment. The apoptotic neurons of the striatum were detected by terminal deoxyncleotidyl transferase-mediated dUTP-biotin nick end labeling assay. Results In comparison with the normal control group, the pole test score and apoptotic neuronal percentage were increased significantly （P〈0.01）, and the locomotor count, swimming test score were decreased remarkably in the model group （P〈0.01）. Compared with the model group, the locomotor counts and swimming test scores were increased obviously （P〈0.05, P〈0.01 ）and the pole test scores decreased apparently （P〈0.05） in both acupuncture and medication groups after the treatment. No significant differences were found between the acupuncture and medication groups in the motor behavior （P〈0.05）. The effect of acupuncture was significantly superior to that of the medication in reducing neuronal apoptosis of the striatum （P〈0.01）. Conclusion Acupuncture of ＂Baihui＂ （GV 20）, ＂Fengfu＂ （GV 16） and ＂Yanglingquan＂（GB 34） can improve PD mice＇s locomotor function, which is closely associated with its function in reducing neuronal apoptosis of the striatum.
出处
《针刺研究》
CAS
CSCD
北大核心
2012年第3期186-190,共5页
Acupuncture Research
基金
国家"973"计划(No.2009CB 522901)
国家中医药管理局重点学科建设项目
上海市卫生局青年科研项目(No.2009Y084)
上海中医药大学科学技术研究发展基金项目(No.2009031)
关键词
帕金森病
针刺
细胞凋亡
运动功能
纹状体
Parkinson disease
Acupuncture
Neuronal apoptosis
Motor function
Striatum
