摘要
为了解含十字形结构域蛋白-3(JMJD3)对肺癌细胞A549增殖与迁徙的影响,通过JMJD3表达质粒转染A549培养48h后,EDU染色及流式细胞术检测细胞增殖与凋亡,划痕实验检测细胞迁徙,RT-PCR检测p21mR-NA表达。结果显示,JMJD3转染后EDU阳性比例明显高于对照(JMJD3:40.75%±2.07%,对照:20.97%±1.5%,P<0.001);G1期细胞比例降低(JMJD3:47.80%±1.85%,对照:54.60%±0.95%,P=0.005)。转染JM-JD3后p21mRNA表达量为35.89%±3.71%,而对照为91.78%±3.74%,两组p21表达变化明显(P<0.001)。转染JMJD3质粒24h和48h后细胞迁徙距离分别为(0.47±0.27)cm和(0.96±0.40)cm,对照为(0.57±0.22)cm和(1.08±0.33)cm,两组无显著性差异(P>0.05)。JMJD3可促进A549细胞增殖,降低G1期细胞比例并下调p21mRNA表达,但对A549迁徙无明显影响。
For investigating the effect of Jumonji domain-containing protein-3 （JMJD3） on the behavior of lung cancer cell line, A549 proliferation was measured with EDU staining and flow cytometer after JMJD3 expression plasmid and pcDNA3.1 transfection at 48h. The migration ability of A549 was tested at the same time. The expression of p21 mRNA was measured with RT-PCR. The results showed that JMJD3 transfection increased the EDU positive cells ratio （J MJD3：40.75% ± 2.07% ,control： 20.97 %± 1.5 %, P〈0. 001）. G1 phase cell ration also increased af- ter J MJ D3 transfeetion（J MJ D3 ： 47.80%± 1.85 %, control ： 54.60 %±0.95%, P = 0. 005 ）. The mRNA expression of p21 decreased inJMJD3 group （JMJD3： 35.89%±3.71%,control： 91.78%±3.74%, P〈0.001）. The distances of migration were （0. 47±0.27）cm and （0.96±0. 40）cm after 24h and 48h with JMJD3 tranfection, compared to （0.57±0.22）cm and （1.08±0.33）cm in control, respectively （P〉0.05）. JMJD3 promoted the prolifera tion of A549 and decreased the G1 cell numbers, decreased the p21 mRNA, but had no effect on A549 migration.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2012年第3期514-518,共5页
Journal of Biomedical Engineering
基金
国家自然科学基金资助项目(30500222
30971325
30871118)
教育部新世纪优秀人才资助项目(NCET-08-0374)
四川省科技厅杰出青年基金资助项目(09ZQ026-020
2009SZ0190)
关键词
含十字形结构域蛋白-3
肺癌
细胞增殖
细胞迁徙
Jumonji domain-containing protein-3 （JMJD-3）
Lung cancer
Cell proliferations Cell migration
