摘要
目的:探讨胺碘酮对SD大鼠甲状腺组织的激素分泌、信号蛋白表达及形态结构等影响。方法:通过对胺碘酮低剂量组(50mg.kg-1.d-1)及高剂量组(200mg.kg-1.d-1)的SD大鼠14周的饲养,取静脉血测游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)、总蛋白(TP)、甘油三酯(TG),取甲状腺组织观察Bcl-2蛋白的表达及Caspase-3蛋白活性高低,并进行组织学检查。结果:用药后SD大鼠外观出现脱毛、行动迟缓、体重下降,血清总蛋白下降;FT3随胺碘酮剂量的增大而下降,FT4在低剂量组略有增高,而在高剂量组则下降;Bcl-2蛋白表达在用药组均增强,而凋亡效应因Caspase-3活性无差异。结论:胺碘酮对SD大鼠甲状腺的影响主要表现为甲状腺激素代谢受抑制,且随剂量的增大而明显,此时甲状腺组织Bcl-2蛋白表达增强,而Caspase-3细胞凋亡效应因子尚未启动。
To investigate the effects of amiodarone on thyroid hormone secretion, sig- naling protein expression and morphology. METHODS： SD rats were divided into low-dose group （50 mg·kg^-1·d^-1） and high-dose group （200 mg·kg^-1·d^-1）, feeding amiodarone for 14 weeks respectively. Free triiodothyronine （FT3）, free thyroxine （FT4）, total protein（TP）, glycerin three greases （TG） were meas- ured from SD rats‘ blood. Analysis of the ex- pression for Bcl-2 protein and the activity of Caspase-3 protein were observed from SD rats’ thyroid gland, and pathology examination were performed. RESULTS：After amiodarone medica- tion, SD rats‘ appearance were hair removal, sluggish, weight loss, serum total protein de-creased, FT3 decreased with amiodarone＇s dose increasing, FT4 in the low-dose group slightly increased, while in the high-dose group de- creased. The positive expression of Bcl-2 in low- dose group and high-dose group, while negative expression of Caspase-3 in all of them （P 0.05）. CONCLUSION= Effects of amiodarone on SD rats’ thyroid were manifested as thyroid hor- mone metabolism were inhibited with the dose increasing, and the expression of Bcl-2 protein were enhanced, while the apoptosis by Caspase- 3 induced had not been initial.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2012年第5期508-512,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
浙江省科技厅公益性技术应用研究计划项目(2010C33008)
浙江省嘉兴市科技计划项目(2010AY1036)
