摘要
首次把生长特性有显著差异的 2株甲型肝病毒 (L8株、H2 株 )的蛋白酶 2A基因分别用基因工程技术克隆到大肠杆菌表达载体 pGEX -KG上 ,重组质粒转化至大肠杆菌 (E .coliBL - 2 1和DH5α)中 ,表达的结果显示 :2株甲型肝病毒的蛋白酶 2A均得以表达 ;表达量占菌体总蛋白 2 0 %以上 .为进一步纯化该蛋白酶、研究其生物学功能和与真核细胞代谢的关系奠定了基础 .
The genes encoding the viral protein enzyme 2A of different strains (L 8 and H 2) of hepatitis A virus were cloned in E.coli DH5α and expressed in E.coli BL-21,the results showed that the genes for 2A of L 8 and H 2 strains were expressed completely,the expressed amount of 2A accounted for 20% of the total amount of E.coli proteins.The research provided the basis for further studies on relation between function of 2A and metabolism of cells.
出处
《云南大学学报（自然科学版）》
CAS
CSCD
2000年第2期141-143,共3页
Journal of Yunnan University(Natural Sciences Edition)
