摘要
目的:建立沙门菌实时荧光PCR的快速检测方法,探讨其可行性和应用价值。方法:根据沙门菌的特异性DNA作为靶序列,以沙门菌菌株提取核酸DNA作为模板进行荧光检测。结果:本研究在7种相关菌株的检测中,除沙门菌出现很好的阳性外,其余菌株均为阴性;在纯菌条件下,定量检测低限为30 cfu/ml;同一样品检测3次Ct值的变异系数均小于5%;对模拟标本与分离培养对比,二者符合率为100%。结论:该方法的建立,不仅为食源性沙门菌污染及食物中毒的快速检测提供依据,而且还可直接用于临床粪便标本的检测。
Objective：To establish a real-time fluorescence quantitative PCR method for rapid detection of Salmonella and investigate the feasibility and application value.Methods： Based on Salmonella specific gene,DNA extracted from Salmonella strain was used as template for fluorescence detection.Results： Results of all 7 bacteria strains were negative except for strains of Salmonella.The detection limit of the method was 30 cfu/ml with pure cultures of Salmonella.Coefficient variables were all less than 5% in 3 different detections of the same sample.To compare simulation specimens with culture strains,coincidence rate was 100%.Conclusion： This method can provide the basis for rapid detection of food-borne contamination of Salmonella and direct clinical stool specimens.
出处
《中国卫生检验杂志》
北大核心
2012年第5期1071-1073,共3页
Chinese Journal of Health Laboratory Technology
