摘要
目的:探讨乙型肝炎表面抗原定量检测阳性标本进行确认实验的研究。方法:收集化学发光微粒子免疫分析法(CMIA)检测HBsAg阳性标本1974例,应用酶联免疫吸附试验(ELISA)等方法复检,对ELISA法末检出的标本进行CMIA法中和确证实验。结果:1974例标本使用ELISA法检测HBsAg有漏检现象的发生,漏检率为9.1%。漏检标本经CMIA法中和确证实验阳性率为96.67%,有6例无法确认。结论:定性方法在HBsAg检测上与发光等定量方法相比存在明显不足。对低浓度HBsAg阳性者应通过中和试验等予以确认。
Objective：To investigate the study of confirmatory test for hepatitis B surface antigen（HBsAg）-positive samples detected by quantitative experiment.Methods： 1974 positive samples tested by chemiluminescent micropaticle immunoassay（CMIA） were collected and the HBsAg were detected by enzyme-linked immunoasorbent assay（ELISA） and HBsAg ELISA negative samples were confirmed by CMIA neutralization test.Results： 1974 samples had undetected situation by ELISA methods.The missed detection rate was 9.1%.Undetected samples by ELISA were confirmed by CMIA neutralization test and the positive rate was 96.67%.6 cases could not be confirmed.Conclusion： Compared with quantitative methods such as chemiluminescence,the qualitative methods were obviously insufficient.The confirmatory tests were done for the low-concentration HBsAg-positive patients.
出处
《中国卫生检验杂志》
北大核心
2012年第5期1031-1033,1036,共4页
Chinese Journal of Health Laboratory Technology
基金
杭州市萧山区科技局课题(2010702)
