摘要
为探讨湖泊沉积物中的生源要素对细菌含量的影响,采用实时定量PCR技术检测沉积物中的细菌含量,运用统计学方法找出中值粒径、总有机碳、总氮、总磷等环境因子与细菌含量之间的相关关系,并据此对影响细菌含量的环境因子进行分析.结果表明:湖泊沉积物中细菌含量随深度的增加而减少;沉积物粒径影响营养元素的赋存从而对细菌含量产生影响;总氮、总磷、总有机碳对沉积物中细菌含量的影响与湖泊水域环境、不同季节温度有关.
In order to study the influence of biogenic elements on bacterial content in lake sediments, the real-time quantitative PCR technology was employed to examine the bacterial content in the sediments at several sampling points. Statistical analysis was conducted to obtain the correlations between the bacterial content and environmental factors, including the medium diameter, total organic carbon, total nitrogen, and total phosphorus, in order to identify the environmental factors that affect the bacterial content. The results show that the bacterial content in the lake sediments decreased with the increase of the sediment depth; the grain sizes of the sediments had an effect on the occurrence of nutrient elements, and thus affected the bacterial content in the sediments; and the effects of total nitrogen, total phosphorus, and total organic carbon on the bacterial content in the sediments were correlated with the water environment of lakes and the temperature in different seasons.
出处
《河海大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第3期275-280,共6页
Journal of Hohai University(Natural Sciences)
基金
国家自然科学基金(50879018)
水利部公益性行业科研专项(201001030)
国家重点实验室专项经费(1069-50987112)
关键词
湖泊沉积物
细菌含量
实时定量PCR
影响因子
lake sediments
bacterial content
real-time quantitative PCR
influencing factor
